In Vitro Propagation of <I>Oxalis corymbosa</I> D.C.

Abstract

In vitro propagation of Oxalis corymbosa D.C. could be carried out via direct and indirect organogenesis. Direct organogenesis was induced by culturing 0.5 cm petiole on basal Murashige and Skoog (MS) medium supplemented with 1.0 mg/l indole-3-acetic acid (IAA) and 0.1 mg/l N-phenyl-N-(1,2,3-thidiazol-5-yl) urea (thidiazuron or TDZ) for 4 weeks. At the end of culture, a number of shoot of 3.30 shoots/explant was obtained. Indirect organogenesis could be induced on MS supplemented with 1.0 mg /l IAA and 5.0 mg/l 6-furfurylaminopurine (KN) after culture for 4 weeks. On that culture, 100% of the callus was obtained. For induction of shoots, the callus must be transfered to MS medium supplement with 1.0 mg/l  IAA and 0.1 mg/l TDZ and culture for 4 weeks. By this method, 6.20 shoots/callus were obtained. Shoot proliferation was carried out by excision single shoot and transfered to culture on MS supplement with 0.1 mg/l TDZ for 4 weeks. An average of 4.27 shoots was induced from one shoot. Root induction could be done by excision single shoot and cultured onto MS supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 0.5 mg/l naphthalene-1-acetic acid (NAA) for 3 weeks. Rooted shoots were successfully trasferred to soil under both cover and non-cover plastic cup.