Development of Culture Media and Yield Comparison of Pleurotus Mushroom Hybrids
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Abstract
Suitable culture media for growing oyster mushroom hybrid cultivar KDCM-4 was studied. It was found that Pararubber tree and Kapok tree sawdust at the ratio 1:1, 1% lime, 1.5% of MgSO4, 20% rice bran and 74% moisture content of the culture media gave the highest yield. When this mixture of culture media (Formula 1) were compared with another media (Formula 2) of the same ratio of sawdust, 1% lime 0.2% MgSO4, 10% rice bran and 70% of moisture content of the media. It was found that Formula 1 gave higher yield.
Yield potential of 5 Pleurotus mushroom hybrids were compared with their parents KD1 from Japan and CM1 stock of the Department of Horticulture CMU. CM1 gave the highest yield followed by KDCM-4. Electrophoretic isozyme assays of esterase, peroxidase and acid phosphatase were conducted on 7 strains on 8.5% polyacrylamide gel. The results indicated that the enzyme pattern of hybrids have the relation with their parents. Most of enzyme bands of the Pleurotus mushroom hybrids were similar to Pleurotus ostreatus from Japan (KD1)
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References
Deniel J.R. and M. Bernie. 1982. Use of isozyme variation to identify genotypic classes of Agaricus brunnescens. Mycology Res. 100(6) 717-731.
Gerrits, J.P.G. 1959. The influence of water in mushroom compost. Mushroom Sci. (4)
Kalberer, P. and U. Kunsch. 1974. Amino acid composition of the oyster mushroom (Pleurotus ostreatus). Lebensm-Wiss. U. Technol. 7: 242-244.
Molitoris, H.P. 1978. Wood degradation, phenoloxidase and chemotaxonomy of higher fungi. Mushroom Sci. X: 243-236.
Ranjhan, S., J.C. Glaszmann., D.A.Ramirez and G.S Khush. 1987. Chromosomal location of four
isozymes loci by trisomic analysis in rice (Oryza sativa L. ). Theor. Appl. Genet. 75: 541-545. Song C.H., K.Y. Cho. 1989, Growth stimulation and lipid synthesis in Lentinus edodes. Mycologia. 81 (4): 514-522.
Tan, K.K. 1981. Cultivation of the oyster mushroom Pleurotus ostreatus, on cotton waste. Mushroom Sci. 6: 697-703.
Visscher, H.R. 1987. Supplementation of the substrate for Pleurotus species at filling. Mushroom Sci. 7(Part 2): 229-240.
Zadrazil F. 1974. The ecology and industrial production of Pleurotus ostreatus, Pleurotus florida, Pleurotus comucopiae, and Pleurotus eryngiï. Mushroom Sci. 4 (Part 1): 621-652.
Zervakis, G. J. Sourdis and B. Constantinos. 1994. Genetic variability and systematics of eleven Pleurotus species based on isozyme analysis. Mycology Res. 98(3): 329-341.