Milk Extender Usage for Deep Frozen Semen

Abstract

Effect of milk extenders had been studied by compared with Egg yolk-tris-glycerol (ETG) which was the standard semen extender used in deep frozen semen process. Semen from three Holstein Friesian bulls were extended in four semen extenders which were Egg yolk-tris-glycerol (ETG), Skim milk-glycerol (SMG), Pasteurized whole milk-glycerol (PMG), and UHT whole milk-glycerol (UMG). Final extender in all semen contained 7% glycerol. Extended semen was placed in 0.25 ml. French mini straws and frozened. Post thaw motility (PTM) had been evaluated twice, immediately post thaw (0 h) and again after 5 h of 37 °C incubation. At 0 h ETG and UMG (36.76 ±1.69% and 35.49 + 1.69%) were significant superior (P<0.05) than SMG and PMG (23.54 ±1.69% and 24.85 ±1.69%). At 5 h evaluation ETG, SMG, PMG, and UMG were varied (P<0.05) by which ETG was greatest (13.62 + 1.38%) along with UMG (9.26 + 1.38%) which were superior than SMG and PMG (5.21 ±1.38% and 2.07 + 1.38%) (P<0.05). Results indicated that UMG could be used as good as of ETG in deep frozen semen process.

Cost of semen extender per dose (0.25 ml. straw) of ETG, SMG, PMG and UMG had been evaluated and were found that they were 0.093, 0.028, 0.030 and 0.034 Baht respectively. In order to use UMG inplace of ETG the cost of frozen semen per dose could be 0.059 Baht reduced.