Toxicity Test of Root Extracted Substances from Vetiver Grass on Diamond Back Moth
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Abstract
Root of vetiver grass Vetiver zizanioides (Linn.) were collected and extracted with steam distillation method. The toxicity of extracted volatile oil was tested with Plutella xylostella L. under laboratory condition using two methods namely Topical application (contact poison) and Feeding method (stomach poison). The two methods were infested with 3rd instar larvae of diamond back moth and observed the mortality daily for 4 days. The result revealed that the highest concentration (100%) killed 37.14% and 51.52% of the larvae with contact poison and stomach poison, respectively. There is no significantly difference of mortality when 40% to 90% concentration were applied. The concentration less than 30% has no effect on mortality of larvae. However, tested larvae were finally weak and died during pupa stage.
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