Effect of BA on Development of Orthotropic Nodes of Arabica Coffee (<I>Coffea arabica</I> L.) <I>in vitro</I> Propagation
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This experiment was aimed to study the effect of a growth regulator: BA, on development of orthotropic nodes of arabica coffee in order to find out rapid vegetative propagation suitable for coffee. Orthotropic nodes of arabica coffee cultivars: LC1662, C1669-31, Progeny88, Progeny90 and Caturra were cultured on MS medium supplemented with BA 0, 0.23 and 2.35 mg/l; or in equivalent 0, 1 and 10 mM; to make MSO, MS1 and MS10 respectively. The result showed that explants cultured on MS10 had better performance in terms of apical and axillary buds development, node development, proliferation and survival rate and average leaf size in every cultivar tested except Progeny88 of which MS1 gave better result. Among various cultivars tested; C1669-31, Caturra and Progeny88 were suitable for orthotropic node propagation in vitro.
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References
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