ANTIBODY PRODUCTION AGAINST HORMONES FOR RADIOIMMUNOASSAY I. COMPARISON AMONG CHICKEN, RABBIT AND GUINEA PIG IN ANTIBODY PRODUCTION AGAINST PROGESTERONE
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Abstract
The current experiment aim to raise antisera against progesterone in chicken, rabbit and Guinea pig by 11 Alpha-Hydroxyprogesterone-hemisuccinate-Bovine Serum Albumin to which self-prepared (Conjugate P) and external source (Conjugate S) were also assessed. The experiment were conducted at Department of Animal Husbandry, Faculty of Agriculture, Chiang Mai University. Nine each of ISA Brown layer, Native x New Zealand White rabbit and Guinea pigs were used. Within each species, 3 animals per group were immunized with Freund's Complete Adjuvant (FCA) only, FCA + 20 microgram Conjugate P, and FCA + 20 microgram Conjugate S by subcutaneous injection at 3 points along the mid-line of the back. Number of immunization were 4 times at 2 weeks interval. The result showed that rabbits were capable of producing the antibody better than chicken and Guinea pig. Rabbit and chicken produced greater amount of antibody when immunized with Conjugate P in comparison with Conjugate S while Guinea pig responded better to Conjugate S. However, there was individual variation within species in the antibody production. The achievement of the present experiment are as: (1) availability of antibody to progesterone for further radioimmunoassay (2) conjugation technique between steriod and protein for future active immunization experiment.
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References
Cox, R.I. and Wilson, P.A. (1976). Effects of immunizing sheep to steriod oestrogens and phyto- oestrogens. J. Reprod. Fert. 46: 524.
Cox, R.I. and Wilson, P.A., Scaramuzzi, R.J., Hoskinson, R.M., George, J.M. and Bindon, B.M. (1982). The active immunization of sheep against oestrone, androstenedione or testosterone to increase twinning. Proc. Aust. Soc. Anim. Prod. 14: 511-514.
Dobson, H. (1983). A Radioimmunoassay Laboratory Handbook. Liverpool University Press, Liverpool.
Erlanger, B.F., Borek, F., Beiser, S.M. and Lieberman, S. (1957). Steriod-protein conjugates. I. Prepa- ration and characterization of conjugates of bovine serum albumin with testosterone and with cortisone. J. Biol. Chem. 228: 713-727.
Erlanger, B.F., Borek, F., Beiser, S.M. and Lieberman, S. (1959). Steriod-protein conjugates. II. Preparation and characterization of conjugates of bovine serum albumin with progesterone, deoxycorticosterone, and estrone. J. Biol. Chem. 234: 1090-1094.
Henricks, D.M. and Mayer D.T. (1977). Gonadal hormones and uterine factors. In: Reproduction in Domestic Animals. Cole, H.H. and Cupps, P.T. (eds.). Academic Press, New York. p. 79-117. International Atomic Energy Agency. (1984). Laboratory Training Manual on Radioimmunoassay in Animal Reproduction. Technical Reports Series No. 233. IAEA, Vienna.
Kamonpatana, M., Timsard, V., Pansin, C. and Cox, R.I. (1985). Steriod immunization in the swamp buffalo: Potential for subfertile recovery. Buffalo J. 1: 53-60.
Lindner, H.R., Perel, E., Friedlander, A. and Zeitlin, A. (1972). Specificity of antibodies to ovarian hormones in relation to the site of attachment of the steroid hapten to the peptide carrier. Steriods 19: 357-375.
Quirke, J.F., Hanrahan, J.P., Tait, A.J. and Kilpatrick, M.J. (1986). Effects of active immunization against androstenedione on ovulation rate and fecundity in Galway and Finish Landrace sheep. Livest. Prod. Sci. 15: 27-37.
Reeves, J.J. (1987). Endocrinolgy of reproduction. In: Reproduction in Farm Animals. Hafez, E.S.E. (ed.). Lea and Febiger, Philadelphia. p. 85-129.
Vaitukaitis, J., Robbins, J.B., Nieschlag, E. and Ross, G.T. (1971). A method for producing specific antisera with small doses of immunogen. J. Clin. Endocr. 33: 988-991.
Zarrow, M.X., Yochim, J.M., Mc Carthy, J.L. and Sanborn, R.C. (1964). Experimental Endocrinology: A Sourcebook of Basic Techniques. Academic Press, New York. p. 65-108.
