IN VITRO MICROTUBERS POTATO PRODUCTION

Abstract

Insect-proof nurseries and the combination of heavy using of insecticides are needed for the virus-free seed potato production, which made quite a high cost of production. Besides these problems, transportation of planting material from the nurseries to the field, in the case of rapid multiplication by using single-node cutting, has to be considered. This experiment was oriented to solve the above difficulties therefore the aseptic microtuber production technique, which needs only small space, easy to transport and storage, and could purely protect the reinfection of viruses, was used. Modified Murashige and Skoog's medium (1962) was used as a basic medium. For the shoot multiplication, medium supplied with IAA0.3 ppm, Adenine sulphate 80 ppm and 2-ip 30 ppm provided the strong and healthy shoots and leaves, but gave the significant lower number of shoot than medium supplied with IAA 0.3 ppm, GA30.1 ppm. and Adenine sulphate 80 ppm. Most of the media used in this experiment, except one which GA3 was added in the shoot multiplication step, could induce the tuberization. Size and number of microtuber in each bottle depended on the age of the plant material and the sucrose concentration. The average number of tubers obtained from this experiment was 4-12 tubers per bottle.