Genetic Analysis of <I>Paphiopedilum myanmaricum</I>
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Abstract
Paphiopedilum myanmaricum is a new species in subgenus Brachypetalum recently discovered in 2017. Leaf and flower characters are very similar to Paphiopedilum josianae. This research applied RAPD (random amplified polymorphic DNA) technique to compare P. myanmaricum with 10 other species in the same subgenus. Thirty RAPD primers were screened, 7 of which were able to amplify clear polymorphic bands with sizes ranging from 180 to 2000 bp. Similarity coefficients were calculated and dendrogram was constructed by UPGMA using NTSYS (version 2.2). The results showed that 11 Paphiopedilum species in subgenus Brachypetalum could be divided into 2 clusters of 4 subgroups with similarity coefficients ranging from 0.400 to 0.850. Genetic variation was in accordance with flower characteristics of each species. Some common flower characters, however, could be identified within each subgroup. Dendrogram analysis revealed close relationship between Paphiopedilum concolor and P. concolor var. hennisianum with the highest similarity coefficients of 0.850 and between P. myanmaricum and P. josianae with similarity coefficients of 0.663. The RAPD results enable genetic assessment of P. myanmaricum among other species in subgenus Brachypetalum. Chromosome study of P. myanmaricum by Feulgen squash method indicated a chromosome number 2n = 26. This information can be applied for plant identification and breeding program.
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References
Chung. S.Y., S.H. Choi, M.J. Kim, K.E. Yoon, G.P. Lee, J.S. Lee and K.H. Ryu. 2006. Genetic relationship and differentiation of Paphiopedilum and Phragmipedium based on RAPD analysis. Scientia Horticulturae 109(2): 153-159.
Cribb, P.J. 1998. The Genus Paphiopedilum. 2nd ed. Natural History Publications (Borneo) Sdn. BHd., Kota Kinabalu, Sabah. 427 p.
Doyle. J.J. and J.L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12(1): 13-15.
Duangkhet. M., N. Potapohn and W. Bundithya. 2014. Genetic relationship analysis of dwarf Doritis pulcherrima Lindl. by RAPD and ISSR techniques. Journal of Agriculture 30(2): 99-109. (in Thai)
Górniak, M., D.L Szlachetko, A.K. Kowalkowska, J. Bohdanowicz and C.X. Canh. 2014. Taxonomic placement of Paphiopedilum canhii (Cypripedioideae; Orchidaceae) based on cytological, molecular and micromorphological evidence. Molecular Phylogenetics and Evolution 70: 429-441.
Iamwiriyakul, P. 2006. Paphiopedilum thaianum, a new species of Paphiopedilum from Thailand. The Orchid Review 114(1271): 278-281.
Koopowitz, H. 2008. Tropical Slipper Orchids: Paphiopedilum and Phragmipedium Species and Hybrids. Timber Press Ins., Portland, Oregon. 411 p.
Koopowitz. H., P. Iamwiriyakul and S. Laohapatcharin. 2017. Paphiopedilum myanmaricum, a new species of slipper orchid (Cypripedioideae, Orchidaceae). Phytotaxa 324(1): 97-100.
Koopowitz. H., P. Iamwiriyakul and S. Laohapatcharin. 2018. Paphiopedilum myanmaricum, a new species of slipper orchid (Cypripedioideae, Orchidaceae). Orchid Digest 82(1): 10-15.
Renz, J. 2011. Renziana Vol.1: Paphiopedilum. Swiss Orchid Foundation. Friedrich Reinhardt Verlag, Basel. 100 p.
Sanpote, P. 2009. Chromosome numbers and karyotypes of some Orchidaceae genus Paphiopedilum in Thailand. Naresuan University Science Journal 6(1): 89-100. (in Thai)
Taywiya. P., W. Bundithya and N. Potapohn. 2008. Analysis of genetic relationship of genus Phalaenopsis by RAPD technique. Acta Horticulturae 788: 39-46.
Thanananta. T., T. Meesangiem and N. Thanananta. 2017. Genetic relationship assessment and identification of Paphiopedilum subgenus Brachypetalum section Brachypetalum Using SCoT markers. Thai Journal of Science and Technology 6(2): 161-170. (in Thai)
