Fungicides Efficacy on Inhibition of Spore Germination and Viability of Strawberry Powdery Mildew
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Abstract
This research aimed to study the efficacy of some fungicides for inhibition of powdery mildew fungi in strawberry plants. Twelve treatments were established using the highest recommended rate of contact fungicides; electrolyzed oxidized water (EOW), sulfur, aliphatic hydrocarbon, sodium bicarbonate, mancozab, and systemic fungicides; azoxystrobin, difenoconazole + azoxystrobin, triforine, metalaxyl, penthiopyrad, and carbendazim. Sterile distilled water was set as a comparing control treatment. After Incubating the fungus spore for 6, 12, 18 and 24 hours in each treatment, consistent results of spore germination and viability inhibitions over tested time were observed. The trials for 24 hours, we found that EOW, sulfur, aliphatic hydrocarbon, mancozeb, difenoconazole + azoxystrobin, triforine, metalaxyl, and penthiopyrad chemicals showed non-statistical significance of the high rate spore germinating inhibition at 99 - 100 %. However, FDA stained treatment for detecting spore viability under inverted fluorescent microscope, EOW, sulfur and aliphatic hydrocarbon treatments revealed non-statistical significance at very high rate to inhibit spore viability as consistent for the results of germinations trials. In case of mancozab, rate of spore germinating inhibition was very high at 99.81 %, but low rate at 54.43 % of spore viability inhibition was determined. Although sodium bicarbonate and metalaxyl showed high percentage of spore germinating inhibition at 78.35 and 86.01 % respectively, the ability for inhibiting spore viability was not sufficient. This results revealed that some fungicides effectively inhibited spore germination of powdery mildew fungus but could not successfully suppress the disease due to ability to inhibit spore viability was not sufficient.
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