Comparative Levels Inoculation of <I> Pediococcus pentosaceus </I> for Ensiled Total Mixed Ration on Fermentation Quality, Nutritive Values and In Vitro Digestibility
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Abstract
The objective of this study was to compare the levels of Pediococcus pentosaceus for ensiled total mixed rations (eTMR) production on fermentation quality, nutritive values, and rumen fermentation characteristics by using in vitro gas production technique. The experimental design was a completely randomized design (CRD) with 5 treatments and 3 replicates, 1) fresh total mixed ration (fresh TMR), 2) eTMR + no P. pentosaceus, 3) eTMR + P. pentosaceus 104 CFU/g (PP4), 4) eTMR + P. pentosaceus 105 CFU/g(PP5) and 5) eTMR + P. pentosaceus 106 CFU/g (PP6), respectively. Comparison of fresh TMR and eTMR had shown that ensiling process can reduce pH values, increase NH3-N, lactic acid, and butyric acid concentration significantly difference among treatments. In addition, it was significantly decreased proportion of EE, NDF, hemicellulose and accumulative gas production at 72 hours while, using P. pentosaceus inoculation were significantly reduce pH values and butyric acid concentration when compare with eTMR. Moreover, using P. pentosaceus inoculation can enhance in vitro gas production and increase IVDMD and IVOMD of eTMR. The different levels of P. pentosaceus inoculation can significantly affect on pH values and NH3-N concentration. It was found that at PP6 inoculation has shown the lowest of NH3-N concentration. However, the levels of P. pentosaceus inoculation shown similar results in nutritive values, IVDMD and IVOMD. Under this study, it could be concluded that P. pentosaceus inoculation at 104 to 106 CFU/g can be used for eTMR production, the highest quality of eTMR fermentation in terms of the lowest pH and NH3-N concentration can obtain by using P. pentosaceus inoculation at 106 CFU/g.
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