The Measurement of Volatile fatty acid concentration and pH value in Rumen Fluid of Dairy cow by Near-Infrared Spectroscopy technique

Abstract

pH value and volatile fatty acid (VFA) measurement in ruminal fluid sample is a standard laboratory method to diagnose rumen acidosis in dairy cow. The previous studies showed that VFA measurement in various samples by using Near-infrared spectroscopy (NIRS) technique is a reliable to implement as analytical instrument. The objectives of this study were to assess the relationship between laboratory VFA concentration and pH value in ruminal fluid samples of dairy cow compare to VFA concentration and pH value in ruminal fluid samples of dairy cow by applied NIRS technique for generating the calibration equation. One-hundred and twenty eight of ruminal fluid samples from fistulated cross-bred Holstein-Freshian dairy cow with ruminal acidosis induction were collected. All samples were analyzed for VFA concentration and pH value by using Gas Chromatography-Mass Spectrometry (GC-MS) and portable pH meter, respectively. NIRS was used for analyzed and generated calibration equation. The results demonstrated that the mean concentration of VFA and pH were 105.82±39.74 and 5.4±0.44, respectively. The statistical analysis to predict VFA concentration and pH value demonstrated the R value were 0.93 and 0.85, respectively. The application of NIRS to analyze VFA concentration and pH value in this study reveal the moderate reliable for study and research scale, thus more data collections are required for higher accuracy of the analysis.