Screening of Lipase-producing Yeasts from Canteen Wastewater: Their Applications in Wastewater Treatment and Biodiesel Production

Abstract

Total 74 yeast isolates were screened for lipase production from the oil contaminated in soil and water in canteen of Rajabhat Maha Sarakham University using the enrichment medium supplemented with olive oil as a sole carbon source. There isolates (CTWY29, CTWY11 and CTWY02) were evaluated highest clear
zone on Tween agar plate after cultured on the Tween agar plate, gave 12, 8 and 6 mm., respectively. The lipid decomposition testing in synthetic wastewater and canteen wastewater were 1.431 and 1.261 Unit/ml obtained CTWY29 and efficiency lipid digested 72.69% in canteen wastewater after 96 hours, Furthermore lipase-producing yeast can be release 3 methyl ester was found that methyl palmitate, methyl linoleate and methyloleate were 20.46, 5.22 and 9.30%,respectively. The results demonstrate that CTWY29 is interesting for potential of lipase production in order to biodiesel at the optimized for laboratory level.