Anaesthetic Effects of Piper Betle Extracts on Nile Tilapia, Oreochromis niloticus

Abstract

Study on Piper betle extracts as an alternative herbal anaesthetic in Nile tilapia, Oreochromis niloticus (1.44±0.20 g in BW and 4.39±1.97 cm in TL). Acute toxicity of Piper betle extracts with LC50 valve was 40.25 ml/L for 24 hr at 95% confidence intervals from 36.49-44.29 ml/L. Piper betle extracts
concentrations were tested at 0, 5, 10, 20, 30 and 40 ml/L. The result showed that the concentration of 30 ml/L was suitable as anaesthtic because tested fish began to show stage 1 (sedation) within 5 min after exposure and fish began to died in 6 hr exposure. The recovery tested fish after exposure in 20, 30 and 40 ml/L were average 4.33±1.52 min.