Biodiesel Production by Oleaginous Yeasts using Corncob Hydrolysate as a Carbon Source

Abstract

In this study, the lipid production potential of 4 oleaginous yeast strains were BJMK02, CTWY07, CTWY19 and SCMK77 in corncob hydrolysate medium under nitrogen limited condition were investigated. Corncob was treated by dilute sulfuric and phosphoric acid. The hydrolysate contained initial 182.32 mg/L of total sugar and reducing sugar 2.34 mg/L. The hydrolysate, after being detoxified by over liming and absorption with activated charcoal, was use for oil production using 4 oleaginous yeast strains. After 144 hour growth in shake - flasks, the CTWY07 had highest lipid content 34.43% of dry cell weight compared with other oleaginous strains. The fatty acid composition of CTWY07 obtained palmitic acids and oleic acids were the major fatty acid. Corncob thus is a promising raw material for microbial oil
production by oleaginous microorganism and application for biodiesel renewable.