Cellulose extraction from cyanobacteria Nostoc sp. for alternative energy source

Abstract

This research was to study cellulose extraction from cyanobacteria, Nostoc sp. One gram of dried cyanobacteria cell was subjected to 4 steps cellulose extraction process with different solvent concentrations: 1) chlorophyll removal using 25, 50, 70, and 100%(v/v) ethanol, 2) lipid removal using 25, 50, 70, and 100%(v/v) hexane, 3) protein removal using 5, 10, 15 and 20%(w/v) sodium hydroxide and 4) bleaching process using 10, 20, and 30%(v/v) hydrogen peroxide. The results showed that the optimal solvent concentration of cyanobacteria Nostoc sp. cellulose extraction process was 100%(v/v) Ethanol, 100%(v/v) hexane, 10%(w/v) sodium hydroxide, and 30%(v/v) hydrogen peroxide with the average yield of 78.92%. Cyanobacteria Nostoc sp. cellulose was digested with cellulase and subjected to determine reducing sugar contents. The results showed that Nostos sp. contains the reducing sugar contents 27.45 mg/g cellulose. This finding suggested that cyanobacteria Nostoc sp. cellulose could digest to be reducing sugar could use as renewable energy source.