Effects of sucrose and mannitol on in vitro slow growth of Dendrobium cretaceum Lindl.
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This study investigated the in vitro growth retardation of Dendrobium cretaceum Lindl. by adding different concentrations of sucrose and mannitol (0, 2, 4, 6, 8, and 10%) to a Modified Vacin and Went (1949) medium. The experiment was arranged in a Completely Randomized Design (CRD) with six treatments and ten replications. After 24 weeks of culture, the results showed that the medium supplemented with 10% sucrose resulted in the lowest average pseudobulb height (1.51 cm). There were no statistically significant
differences among the sucrose treatments in terms of average pseudobulb diameter (0.23 to 0.25 cm) and the average number of leaves (4.05 to 4.75 leaves). For the mannitol treatments, supplementation with 8% and 10% mannitol produced the lowest average pseudobulb heights (0.79 and 0.72 cm, respectively); however, these concentrations also caused yellowing of leaves and pseudobulbs. While different mannitol concentrations had no significant effect on the average pseudobulb diameter (0.22 to 0.25 cm), they did result in statistically significant differences in the average number of leaves (3.45 to 4.90 leaves). Therefore, using 10% sucrose was more effective than using mannitol for the in vitro growth retardation of Dendrobium cretaceum Lindl.
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กลุ่มงานประสานงานและเสริมสร้างความร่วมมือระหว่างประเทศ กองคุ้มครองพันธุ์สัตว์ป่าและพืชป่าตาม
อนุสัญญา กรมอุทยานแห่งชาติ สัตว์ป่า และพันธุ์พืช. (2556). กล้วยไม้ป่าในผืนป่าตะวันออก ตอนที่ 1 [Wild orchids of the eastern forest, Part 1] (1st ed.). ชุมนุมสหกรณ์การเกษตรแห่งประเทศไทย.
จุฑามาศ วงศ์จักร. (2563). ฤทธิ์ทางชีวภาพของสารสกัดจากกล้วยไม้สกุลหวายต่อการต้านอนุมูลอิสระ และการยับยั้งการเจริญของเซลล์มะเร็งลำไส้ [Biological activity of Dendrobium orchid extracts on antioxidant activity and colon cancer cell growth inhibition] [Master's thesis, Maejo University].
พรพรรณ สุขุมพินิจ, กมรภัทร ศิริพงษ์, และอดิศักดิ์ การพึ่งตน. (2563). อิทธิพลของน้ำตาลซูโครสต่อการชะลอการเจริญเติบโตต้นอ่อนกล้วยไม้เอื้องดอกมะเขือในสภาพปลอดเชื้อ [Effect of sucrose on the in vitro growth retardation of Dendrobium aphyllum seedlings]. วารสารวิจัยรำไพพรรณี, 14(1), 52–56.
มูลนิธิกล้วยไม้ไทย. (2559). ชีวิตงามกล้วยไม้ไทย [The beautiful life of Thai orchids]. เชียงใหม่โรงพิมพ์แสงศิลป์.
รังสฤษดิ์ กาวีต๊ะ. (2545). การเพาะเลี้ยงเนื้อเยื่อพืช: หลักการและเทคนิค [Plant tissue culture: Principles and techniques] (3rd ed.). สำนักพิมพ์มหาวิทยาลัยเกษตรศาสตร์.
AlMousa, R., & Hassan, N. (2022). Effect of osmotic regulators on in vitro conservation of grape. Journal of Genetic and Environmental Resources Conservation, 10, 60–66.
Ashmore, S. E. (1997). Status report on the development and application of in vitro techniques for the conservation and use of plant genetic resources. International Plant Genetic Resources Institute.
Bhuiyan, M., Hossain, M., & Haque, M. (2016). In vitro conservation of taro (Colocasia esculenta var. globulifera) as influenced by mannitol. Bangladesh Journal of Agricultural Research, 41, 67–74. https://doi.org/10.3329/bjar.v41i1.27668
Distabanjong, K., Distabanjong, C., & Ruengwiset, B. (2010). In vitro propagation and conservation of Rhynchostylis gigantea (Lindl.) Ridl. In Proceedings of the 48th Kasetsart University Annual Conference (pp. 571–578). Kasetsart University.
Engelmann, F. (1991). In vitro conservation of tropical plant germplasm — A review. Euphytica, 57, 227–243.
Grout, W. V. (1991). Conservation in vitro. Acta Horticulturae, 289, 171–178.
Hernández-Leal, E., Reyes-López, D., Castillo-Martínez, C. R., Avendaño-Arrazate, C. H., Corona-Torres, T., García-Zavala, J. J., & Pascual-Ramírez, F. (2020). In vitro conservation of Vanilla planifolia hybrids in minimal growth conditions. Agro Productividad, 13, 3–10.
Linjikao, J., Inthima, P., Limmongkon, A., & Kongbangkerd, A. (2024). Mannitol and sorbitol concentration optimization for effective Epipactis flava Seidenf. in vitro slow growth storage. In Vitro Cellular & Developmental Biology – Plant, 60, 496–507. https://doi.org/10.1007/s11627-024-10437-w
Lopez-Puc, G. (2013). An effective in vitro slow growth protocol for conservation of the orchid Epidendrum chlorocorymbos Schltr. Tropical and Subtropical Agroecosystems, 16, 61–68.
Razdan, M. K. (1994). An introduction to plant tissue culture. Chapman Enterprises. (corrected from "Chaman Enterprises" — likely a typo; please verify)
Sarkar, D., & Naik, P. S. (1998). Factors affecting minimal growth conservation of potato microplants in vitro. Euphytica, 102, 275–280.
Sukhumpinij, P. (2019). Effect of sucrose on in vitro germplasm preservation in Dendrobium friedericksianum Rchb.f. Acta Horticulturae, 1262, 63–66.
Towill, L. E. (1995). Cryopreservation by vitrification. In B. Grout (Ed.), Genetic preservation of plant cells in vitro (pp. 99–111). Springer-Verlag.
Vacin, E. F., & Went, F. W. (1949). Some pH changes in nutrient solutions. Botanical Gazette, 110, 605–613.
Zhang, G. N., Zhong, L. Y., Bligh, S. A., Guo, Y. L., Zhang, C. F., Zhang, M., Wang, Z. T., & Xu, L.-S. (2005). Bi-bicyclic and bi-tricyclic compounds from Dendrobium thyrsiflorum. Phytochemistry, 66, 1113–1120. https://doi.org/10.1016/j.phytochem.2005.04.001
