Effect of Silver Nitrate on In vitro Flowering Induction and Prolong Duration of Miniature Rose (Rosa chinensis Jacq)
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Abstract
Rosa chinensis Jacq is a beautiful cut flower that is widely grown for commercial purpose. In this study, nodal explants from three lines (colors) of miniature rose including; red, pink and ocher color were used. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 1, 2 and 3 mg/l Benzyladenine (BA). After 3 months of culture, nodal explants from red color flower line cultured on MS medium supplemented with 3 mg/l BA gave the highest survival rate at 93.3%, multiple shoot formation at 100% and average numbers of shoot at 2.75 shoots/explant. Shoots obtained from previous experiment were excised as individual and transferred to culture on MS medium supplemented with 0, 0.5, 1, 2 and 4 mg/l silver nitrate (AgNO3). After 3 months of culture, shoot of red color flower line were cultured on MS medium supplemented with 4 mg/l AgNO3 gave the highest in vitro flowering formation at 80%. Flowering duration could be prolonged for 44 days.
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