The aim of this research was to screen an effective biosurfactant - producing bacteria isolated from used lubricating oil contaminated soil collected from garage area in Phuket Rajabhat University by using banana peel as a carbon source on Minimal Salt Medium (MSM). Seven bacterial isolates were selected from 159 isolates by using drop collapsing test and emulsification activity. All seven isolates had high activity by drop collapsing test, but they do not exhibit emulsification activity with palm oil. Gram - staining and cell morphology study revealed that all seven isolates were Gram - positive and rods shape. Bacterial isolate 180 showed the highest biosurfactant activity by drop collapsing test (8.50 mm). The optimized conditions for biosurfactant production of bacterial isolate 180 was performed in MSM supplemented with banana peel as a carbon source. The optimized condition was obtained when supplemented with 1% banana peel as a carbon source and 0.1% yeast extract as a nitrogen source incubated at 200 rpm at room temperature (30±5ºC). It exhibited the highest biosurfactant activity up to 8.00 mm after 12 hours of incubation.
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