การประยุกต์ใช้เปลือกกล้วยเป็นแหล่งคาร์บอนสำหรับการผลิตสารลดแรงตึงผิวชีวภาพจากแบคทีเรียที่คัดแยกได้จากดินที่ปนเปื้อนน้ำมันหล่อลื่นเครื่องยนต์ที่ใช้แล้ว
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Abstract
The aim of this research was to screen an effective biosurfactant - producing bacteria isolated from used lubricating oil contaminated soil collected from garage area in Phuket Rajabhat University by using banana peel as a carbon source on Minimal Salt Medium (MSM). Seven bacterial isolates were selected from 159 isolates by using drop collapsing test and emulsification activity. All seven isolates had high activity by drop collapsing test, but they do not exhibit emulsification activity with palm oil. Gram - staining and cell morphology study revealed that all seven isolates were Gram - positive and rods shape. Bacterial isolate 180 showed the highest biosurfactant activity by drop collapsing test (8.50 mm). The optimized conditions for biosurfactant production of bacterial isolate 180 was performed in MSM supplemented with banana peel as a carbon source. The optimized condition was obtained when supplemented with 1% banana peel as a carbon source and 0.1% yeast extract as a nitrogen source incubated at 200 rpm at room temperature (30±5ºC). It exhibited the highest biosurfactant activity up to 8.00 mm after 12 hours of incubation.
Article Details
เนื้อหาและข้อมูลในบทความที่ลงตีพิมพ์ในวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราช ถือเป็นข้อคิดเห็นและความรับผิดชอบของผู้เขียนบทความโดยตรง ซึ่งกองบรรณาธิการวารสารไม่จำเป็นต้องเห็นด้วยหรือร่วมรับผิดชอบใด ๆ
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการตีพิมพ์ในวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราช ถือเป็นลิขสิทธ์ของวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราช หากบุคคลหรือหน่วยงานใดต้องการนำข้อมูลทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อการกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราชก่อนเท่านั้น
The content and information in the article published in Wichcha journal Nakhon Si Thammarat Rajabhat University, It is the opinion and responsibility of the author of the article. The editorial journals do not need to agree. Or share any responsibility.
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