The Efficiency of Bacillus spp. to Remove Ammonia in Shrimp Aquaculture
Salt-tolerant Bacillus was used to eliminate ammonia in shrimp aquaculture wastewater. Bacillus strains were isolated from sediment and water collected from shrimp farms and domestic wastewater. Ammonium oxidizing ability was screened by Griess-Ilosvay method. Five isolates were identified as Bacillus spp. with salt requirement within the range of 0-40 g/L NaCl and an optimal pH of 7. Bacillus strains TS41, TW31, HS12, HW34 and ES33 exhibited preliminary ammonium removal efficiency on HNM medium for 84.21%-94.86%. Improved ability of synthesized shrimp wastewaters was determined by applying 1% and 5% cell suspension for 7 days. SF experiment with 1% cell suspension of ES33 showed highest ammonium removal of 66.38%, while the results of other treatments showed no significance (p > 0.05). SNF experiment, all five Bacillus strains showed ammonium removal of 78-96% at day 7. NSF experiment, ES33 provided the highest ammonium removal efficiency of 1% cell suspension at day 7 for 93.14%. The amounts of nitrite and nitrate were presented in all experiments and removed by Bacillus species. The results demonstrated the process of nitrification-denitrification reaction. Consequently, our Bacillus strains may propose as heterotrophic nitrification-aerobic denitrification species.
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