The Efficiency of Bacillus spp. to Remove Ammonia in Shrimp Aquaculture

  • Thossaporn Phatthongkleang คณะทรัพยากรธรรมชาติ มหาวิทยาลัยสงขลานครินทร์ วิทยาเขตหาดใหญ่
  • Yutthapong Sangnoi
  • Sompong O-Thong
  • Arnon Uppabullung
  • Teeyaporn Keawtawee
Keywords: Salt–tolerant Bacillus, Shrimp aquaculture wastewater, Ammonium removal efficiency, Cell suspension, Nitrification–denitrification


Salt-tolerant Bacillus was used to eliminate ammonia in shrimp aquaculture wastewater. Bacillus strains were isolated from sediment and water collected from shrimp farms and domestic wastewater. Ammonium oxidizing ability was screened by Griess-Ilosvay method. Five isolates were identified as Bacillus spp. with salt requirement within the range of 0-40 g/L NaCl and an optimal pH of 7. Bacillus strains TS41, TW31, HS12, HW34 and ES33 exhibited preliminary ammonium removal efficiency on HNM medium for 84.21%-94.86%. Improved ability of synthesized shrimp wastewaters was determined by applying 1% and 5% cell suspension for 7 days. SF experiment with 1% cell suspension of ES33 showed highest ammonium removal of 66.38%, while the results of other treatments showed no significance (p > 0.05). SNF experiment, all five Bacillus strains showed ammonium removal of 78-96% at day 7. NSF experiment, ES33 provided the highest ammonium removal efficiency of 1% cell suspension at day 7 for 93.14%. The amounts of nitrite and nitrate were presented in all experiments and removed by Bacillus species. The results demonstrated the process of nitrification-denitrification reaction. Consequently, our Bacillus strains may propose as heterotrophic nitrification-aerobic denitrification species.


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Chankaew, S., O-Thong, S. and Sangnoi, Y. (2018). Nitrogen removal efficiency of salt-tolerant heterotrophic nitrifying bacteria. Chiang Mai Journal of Science, 45(1), 11-20.
Charoendat, U., Chumchareon, M. and Phumee, P. (2016). Effects of Creat (Andrographis paniculata Wall. Ex Nees) Extract on Growth Performance and Bacterial Disease Resistance in Pacific White Shrimp (Litopenaeus vannamei Boone). RMUTSV Research Journal, 8(2), 190-202.
Cheng, S.Y. and Chen, J.C. (1999). Haemocyanin oxygen affinity and the fractionation of oxyhemocyanin and deoxyhemocyanin for Penaeus monodom exposed to elevated nitrite. Aquatic Toxicology, 45(1), 35-46.
Dechmahitkul, W., Youkong, C., Poomputra, K., Akeprathumchai, S. and Mekvijitsaeng, P. (2007). Study on media formulation and production process of Bacillus subtilis spores for animal probiotics. KMUTT Research and Development Journal, 30(2), 251-259.
Fennesy, M.S. and Cronk, J.K. (1997). The effectiveness and restoration potential of riparian ecotones for the management of nonpoint source pollution, particularly nitrate. Environmental Science and Technology, 27(4), 285-317.
Gupta, A.B. and Gupta, S.K. (2001). Simultaneous carbon and nitrogen removal from high strength domestic wastewater in an aerobic RBC biofilm. Water Research, 35(7), 1714-1722.
Hmadhloo, S., Tanyaros, S. and Phumee, P. (2013). Effect of C : N ratio in integrated culture of White Shrimp (Litopenaeus vannamei) and Nile Tilapia (Oreochromis niloticus) using biofloc technology. RMUTSV Research Journal, 5(1), 96-106.
Joo, H.S., Hirai, M. and Shoda, M. (2005). Characteristics of ammonium removal by heterotrophic nitrification–aerobic denitrification by Alcaligenes faecalis No. 4. Bioscience and Bioengineering, 100(2), 184-191.
Li, P., Zhang, S. and Liu, D.L. (2005). Study progress of bacterial aerobic denitrification. Microbiology, 25(1), 60-64.
Lin, S.H. and Wu, C.L. (1996). Electrochemical removal nitrite and ammonia for aquaculture. Water Research, 30(3), 715-721.
Lu, Y., Wang, X., Liu, B., Liu, Y. and Yang, X. (2012). Isolation and characterization of heterotrophic nitrifying strain W1. Chinese Journal of Chemical Engineering, 20(5), 995-1002.
Manzo, N., Luccia, D.B., Isticato, R., Apuzzo, D.E., Felice, D.M. and Ric, E. (2013). Pigmentation and sporulation are alternative cell fates in B. pumilus SF214. PLOS ONE, 8(4), 1-12.
Mclntosh, D., Samocha, T.M., Jones, E.R., Lawrence, A.L., Horowitz, S. and Horowitz, A. (2001). Effects of two commercially available low-protein diets (21% and 3%) on water and sediment quality on the production Litopenaeus vannamei in an outdoor tank system with limited water discharge. Aquaculture Engineering, 25(2), 69-82.
Meeboon, N. and Saimmai, A. (2019). Characterization of biosurfactant produced by Bacillus subtilis AS6 isolated from mangrove sediment in Phuket province. Rajamangala University of Technology Srivijaya Research Journal, 11(1), 67-83.
Mevel, G. and Prieur, D. (2000). Heterotrophic nitrification by a thermophilic Bacillus species as influenced by different culture conditions. Canadian Journal of Microbiology, 46(5), 465-473.
Ongsara, N., Sungpud, J. and Liamtong, S. (2012). Microbiological quality of drinking water at Nakhon Si Thammarat Rajabhat University. Wichcha Journal Nakhon Si Thammarat Rajabhat University, 31(2), 11-21.
O-Thong, S., Jiapakdee, R. and Intrasungkha, N. (2003). Wastewater generated from marine shrimp feed and its treatment potential by internal filter system. Thaksin University Journal, 6(1), 41-53.
Paungfoo, C., Prasertsan, P., Burrell, P.C., Intrasungkha, N. and Blackall, L.L. (2007). Nitrifying bacterial communities in an aquaculture wastewater treatment system using fluorescence in situ hybridization (FISH), 16S rRNA gene cloning, and phylogenetic analysis. Biotechnology and Bioengineering, 97(4), 985-990.
Read, P. and Fernandes, T. (2003). Management of environmental impacts of marine aquaculture in Europe. Aquaculture, 226(1-4), 139-163.
Rosovitz, M.J., Voskuil, M.I. and Chambliss, G.H. (1998). Bacillus. Microbiology and Microbial Infections Systematic Bacteriology, 2, 709-729.
Seenivasagan, R., Kasimani, R., Babalola, O.O., Karthika, A., Rajakumar, S. and Ayasamy, P.M. (2017). Effect of various carbon source, temperature and pH on nitrate reduction efficiency in mineral salt medium enriched with Bacillus weinstephnisis (DS45). Groundwater for Sustainable Development, 5, 21-27.
Sirianuntapiboon, S., Chaochon, A. and Chairoung, N. (2015). Efficiency of sequencing batch reactor (SBR) system for treatment of textile wastewater. Wichcha Journal Nakhon Si Thammarat Rajabhat University, 34(1), 1-25.
Song, Z.F., An, J., Fu, G.H. and Yang, X.L. (2011). Isolation and characterization of an aerobic denitrifying Bacillus sp. YX-6 from shrimp culture ponds. Aquaculture, 319, 188-193.
Strickland, J.D.H. and Parsons, T.R. (1972). A Practical Handbook of Seawater Analysis. Canada: Fishery Research Board.
Suharti, H.A. and Vries, S. (2004). NO reductase from Bacillus azotoformans is a bifunctional enzyme accepting electrons from menaquinoland a specific endo genous membrane-bound cytochrome C 551. Biochemistry, 43, 13487-13495.
Sutin, S. (2010). Water quality of mullet (Liza oligolepis, Bleeker, 1985) at Nakhon Si Thammarat bay, Nakhon Si Thammarat province. Wichcha Journal Nakhon Si Thammarat Rajabhat University, 29(2), 58-63.
Usawakesmanee, N. (2016). The Use of dried water hyacinth as a feed supplement for rearing Silver barb (Puntius gonionotus). Wichcha Journal Nakhon Si Thammarat Rajabhat University, 35(1), 70-78.
Vaseeharan, B. and Ramasamy, P. (2003). Control of pathogenic Vibrio spp. By Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Letters in Applied Microbiology, 36(2), 83-87.
Wan, C., Yang, X., Lee, D.J., Du, M., Wan, F. and Chen, C. (2011). Aerobic denitrification by novel isolated strain using as nitrogen source. Bioresource Technology, 102(15), 7244-7248.
Wang, Y.B., Li, J.R. and Lin, J.D. (2008). Probiotics in aquaculture: challenges and outlook. Aquaculture, 281, 1-4.
Yang, X.P., Wang, S.M., Zhang, D.W. and Zhou, L.X. (2011). Isolation and nitrogen removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Bacillus subtilis A1. Bioresource Technology, 102(2), 854-862.
Zhang, Q.L., Liu, Y., Ai, G.M., Miao, L.L., Zheng, H.Y. and Liu, Z.P. (2012). The characteristics of a novel heterotrophic nitrification–aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresource Technology, 108, 35-44.
Zhao, C., Yan, X., Yang, S. and Chen, F. (2017). Screening of Bacillus strains from Luzhou-flavor liquor making for high-yield ethyl hexanoate and low-yield propanol. Food Science and Technology, 77, 60-66.
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