Effects of Diatom (Amphora coffeaeformis) Supplementation in Diet on Growth and Growth-Related Genes Expression in Pacific White Shrimp (Litopenaeus vannamei) Postlarvae ผลของการเสริมไดอะตอม (Amphora coffeaeformis) ในอาหารต่อการเจริญเติบโตและการแสดงออกของยีนที่เกี่ยวข้องกับการเจริญเติบโตในกุ้งขาว (Litopenaeus vannamei) ระยะหลังวัยอ่อน
This experiment aimed to compare study the effects of Amphora coffeaeformis supplementation on the growth performance, survival rate and growth-related genes expression of Pacific white shrimp postlarvae (Litopenaeus vannamei) at different levels. The experiment was designed in 6 treatments using 4 concentrations of A. coffeaeformis supplemented into diet as follows: 0%, 4.31%, 8.26%, 11.89%, 15.25% and live A. coffeaeformis. This diatom was prepared under laboratory conditions and harvested by nylon mesh filter to amass an amount of A. coffeaeformis for drying. The diatom powder was mixed according to the designed treatments. In this study, stage 15 postlarvae (PL15) with the lengths starting from 1.06±0.10 cm. were cultured under specific conditions as follows: salinity 20 psu, temperature 30°C, daily water change (20%), feeding shrimps for 7 times/day and water quality analyzed every 3 days. After culturing postlarvae for 14 days, the results showed that the final lengths of white shrimp postlarvae fed by live and 4.31% supplementation of A. coffeaeformis were the longest sizes of 2.12 and 1.98 cm. comparing to the controlled groups with significantly different final lengths (p < 0.05). Also, the results of growth-related gene expression (Chitinase (CHIT), AMP-activated protein kinase (PRKAG) and protease (Cathepsin) were focused in this study) revealed that white shrimp postlarvae fed with live A. coffeaeformis supplemented diet were the highest growth-related gene expression group compared to the controlled groups with statistically significant difference (p < 0.05). This study indicated that live A. coffeaeformis supplementation affected the highest growth and gene expression of white shrimp postlarvae. It should be noted that no more than 4.31% of dry powder A. coffeaeformis supplementation should be added.
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