Effect of Seed Coating with Streptomyces sp. CU-02 on Quality and Control Damping-Off in Chinese Cabbage (Brassica rapa, subsp. pekinensis)

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Published: Nov 4, 2021
Keywords:
Seed coating seed quality biological control damping-off Streptomyces sp. CU-02

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Nilamai Sansupa
Division of Horticulture, Faculty of Agricultural Production, Maejo University, San Sai, Chiang Mai 50290, Thailand
Jakkrapong Kangsopa
Division of Agronomy, Faculty of Agricultural Production, Maejo University, San Sai, Chiang Mai 50290, Thailand
Pranom Yangkhamman
Chatsuda Phuakjaiphaeo
Division of Plant Protection, Faculty of Agricultural Production, Maejo University, San Sai, Chiang Mai 50290, Thailand

Abstract

Farmers usually use chemical substances to control damping-off in Chinese cabbages which cause contamination risks for farmers and consumers, as well as pollution of the environment. This research focuses on studying the control of damping-off using biological controls to replace the use of chemical substances. The antagonistic microorganisms Streptomyces sp. is used to test its efficiency in promoting growth of Chinese cabbage seeds and controlling damping-off caused by P. aphanidermatum. The test design is based on the completely randomized design (CRD) with four repetitions using five seed coating methods which are 1) uncoated seeds, 2) seeds coated with CMC mixed Streptomyces sp. CU-02 at various concentrations, which is defined as 3) 1x106, 4) 1x107 and 5) 1x108 spore ml-1/10 g. After testing the quality of Chinese cabbage seeds under greenhouse conditions, it was found that Chinese cabbage seeds coated with all levels of Streptomyces sp.CU-02 had better germination, speed of germination index; (SGI), and mean germination time (MGT) compared to uncoated seeds and seeds coated with CMC. Meanwhile, in terms of the efficiency of Streptomyces sp. CU-02 in controlling damping-off, it was found that a 1x106 spore ml-1of Streptomyces sp. CU-02 is efficient in controlling damping-off since a lower percentage of seed rot and higher percentage of healthy seedlings were found compared with other test methods.

Article Details

How to Cite
Sansupa, N., Kangsopa, J., Yangkhamman, P., & Phuakjaiphaeo, C. (2021). Effect of Seed Coating with Streptomyces sp. CU-02 on Quality and Control Damping-Off in Chinese Cabbage (Brassica rapa, subsp. pekinensis). YRU Journal of Science and Technology, 7(1), 57–65. retrieved from https://li01.tci-thaijo.org/index.php/yru_jst/article/view/248707
Issue
Vol. 7 No. 1 (2022): มกราคม-เมษายน
Section
Research Article

บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น

Author Biography

Nilamai Sansupa, Division of Horticulture, Faculty of Agricultural Production, Maejo University, San Sai, Chiang Mai 50290, Thailand

Farmers usually use chemical substances to control damping-off in Chinese cabbages leading to the risk of food contamination and farmers, consumers, as well as pollution of the environment. For studying the control of damping-off using biological controls to replace the use of chemical substances. The antagonistic microorganisms Streptomyces sp. CU-02 was used to test its efficiency in promoting growth of Chinese cabbage seeds and controlling damping-off caused by Pythium aphanidermatum. The test design is based on the completely randomized design (CRD) with four replications using five seed coating methods which were 1) uncoated seeds, 2) seeds coated with Carboxymethyl cellulose (CMC) mixed Streptomyces sp. CU-02 at various concentrations, which is defined as 3) 1x106, 4) 1x107 and 5) 1x108 spore ml-1/10 g. After testing the quality of Chinese cabbage seeds under greenhouse conditions, it was found that Chinese cabbage seeds coated with all levels of Streptomyces sp.CU-02 had better germination, speed of germination index, and mean germination time compared to uncoated seeds and seeds coated with CMC. Meanwhile, in terms of the efficiency of Streptomyces sp. CU-02 in controlling damping-off, it was found that a 1x106 spore ml-1 of Streptomyces sp. CU-02 is efficient in controlling damping-off with a lower percentage of seed rot. Meanwhile efficiency of Streptomyces sp. CU-02 was not evident as significantly increased in percentage damping-off was found that a 1x107 spore ml-1/10 g. high percentage with significance. As a result, 1x106 spore ml-1 of Streptomyces sp.CU-02 is recommended concentrations to coated Chinese cabbages seeds since it is the most cost-effective and had the best inhibitory effect on P. aphanidermatum.

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