Screening of Thermotolerant Amylase-Producing Microorganisms for the Fermented Rice Noodle Wastewater Treatment
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Abstract
Isolation and screening of thermotolerant microorganisms from 18 samples 5 habitats in local places including various areas of rubber factory, charcoal burning plant, rice husk, cow dung and chicken dung that were able to produce amylase used for treating the rice noodle wastewater. The result showed that 15 isolates were thermotolerant bacteria which can grow at 55±2°C but no fungal growth was observed. The qualitative and quantitative of amylase were examined. Six isolates can produce amylase including RF1, RF2, RF4, RF7, RF11 and CBP3. Comparison the enzymatic activity produced by thermotolerant bacteria revealed that thermotolerant bacteria isolate CBP3 could produced the highest activity of amylase ๖(93.5±0.67 U/ml) and specific activity of 80.54 U/ml of protein. Studies on the application of treatment efficiency in rice noodle process effluent in vitro showed that the thermotolerant bacterium isolate CBP3 had BOD and COD removal efficiency of 41.32±0.64 % and 56.23±0.54%. The thermotolerant bacterium isolate CBP3 was identified on the basis of their morphological characteristics showed the CBP3 was classified as Bacillus sp. Therefore, thermotolerant amylase producing bacteria could be apply for wastewater treatment in the fermented rice noodle processing.
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บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
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