Effects of Cytokynins and Concentrations on Development and Number of Multiple Shoots of In Vitro Culture of Caladium bicolor Vent.

Abstract

          The effects of cytokynins and concentrations on development and number of multiple shoots of in vitro culture of Caladium bicolor Vent. using many parts of caladium are apical shoots, leaves, leaves no petiole, leaves with petiole and petioles on semi-solid Murashige and Skoog (MS) medium supplemented with culture media containing cytokinins as BA concentration 3 mg/L, 30 % sucrose and gelatin 1.1%, cultured for eight weeks. The results showed that a parts of apical shoots caladium was the highest a percentage of ability multiple shoots regeneration (85%) and the highest average number of multiple shoots (22.85 shoots). Whereas the four different cytokinins concentrations of 0, 1, 3 and 5 mg/L cultured for eight weeks found that not only the highest percentage of multiple shoots regeneration (100 %) was obtained from media containing BA 5 mg/L but also can produced the highest average number of multiple shoots (18.08 shoots).