Isolation and Screening of Lactic Acid Bacteria from Fermented Food and Application in Bioextract Production for Plant Growth Promotion
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Abstract
Bioextract is composed of microbes such as lactic acid bacteria. These bacteria play a role in nutrient degradation and important substance production. Therefore, the objectives of this research were to isolate and select lactic acid bacteria from fermented food and to characterize and investigate the efficacy of bioextracts from lactic acid bacteria in order to promote morning glory growth. Nine isolates of lactic acid bacteria (PB1-5 and PS6-9) were isolated from fermented food. All lactic acid bacteria isolates (n=9) were investigated for protease and amylase enzymes. The results showed PS6 and PS8 had the highest protease and amylase activities and were used for bioextract production. The bioextract production of 8 formulas was performed using different ratios of lactic acid bacteria and microbial activator (PD.2). The result of the properties investigation of bioextract found that during the period of fermentation, eight bioextracts were brown color, with pH 3.89-6.00, and normal smell without unpleasant odor. The total number of bacteria and lactic acid bacteria in each bioextract during fermentation ranged between 0.07×108 – 3.87×109 CFU/ml and 0.00– 4.37×108 CFU/ml, respectively. In addition, the efficacy study of the bioextracts revealed that formula no. 3 and 4 were able to promote the growth by increasing the number of leaves, leaf length, and also plant height of morning glory. Interestingly, the ability of the bioextract formula no.3 and 4 had no significant differences (p≤0.05) with the bioextract formula no.2 that contained microbial activator PD.2. Therefore, the results of this study indicated that the bioextracts supplemented with lactic acid bacteria isolates from traditional fermented food can enhance plant growth and can further be studied for development as an eco-friendly bioextract.
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บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
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