Effect of Various Ethanol Concentrations on Extracting Crude Yield and Allelopathic Activity of Murraya paniculata Leaf

Abstract

An examination was made of the effects of various ethanol concentrations on the extraction of crude yield and allelopathic activity of Murraya paniculata L. Various water-ethanol concentration (0, 25, 50, 75 and 100%, v/v) was extracted by maceration method on dried leaf of M. paniculata at 1: 10 w/v by three times. The highest crude yield was obtained in 50% ethanol by three times extraction followed by 25%, 0%, 75% and 100% respectively. Each crude extract was tested the allelopathic activity to inhibit the germination and seedling growth of Echinochloa crus-galli and Trianthema portulacastrum L. on the concentrations of 0, 0.25, 0.5, 1 and 2% by petri dish test. The results showed that all crudes did not inhibit seed germination of E. crus-galli and T. portulacastrum, however they effected on seedling survival and seedling growth. The crude of 100% ethanol showed the highest inhibitory effect on root growth of E. crus-galli and T. portulacastrum. The inhibitory level dramatically decreased with reducing on ethanol percentage.