Genetic Diversity of HSP70 Gene in Thai Native Chicken

Abstract

The objective of this study was to investigate the genetic distribution and diversity of HSP 70, in 11 strains of Thai indigenous chicken (Lueng Hang Khao, Pradu Hang Dam, Pha Khoi fighting cock, Burmase fighting cock, Red junglefowl, Chee chicken, Black bone chicken, Commercial laying hens, Commercial broiler, Rhode Island Red, White Leghorn) genotyped by PCR-RFLP technique. The polymorphism of HSP 70 had three genotypes (GG, GC and CC) frequency of allele C than G. The Hardy-Weinberg’s equilibrium (HWE) test found most Thai indigenous chicken population followed HWE except Lueng Hang Khao, Burmase fighting cock, Red junglefowl. The expected heterozygosity (expected heterozygosity; H_E) 0.064-0.473 higher than the observe heterozygosity (observed heterozygosity; H_o) 0.067-0.367. Genetic relationship analysis by using principal component analysis (PCoA) showed the genetic relationship between native chicken population and commercial breed.