Analysis of Genetic Variation of Bactrocera dorsalis (Diptera: Tephritidae) Inferred from mtDNA COI Sequences

Abstract

Population genetic diversity of the oriental fruit fly, Bactrocera dorsalis, in Phitsanulok-Sukhothai areas was assessed using DNA sequences of the mitochondrial cytochrome c oxidase subunit I gene (COI). In total, 16 flies representing two sampled sites were sequenced for a 642-bp fragment of the gene. It was revealed that 10 haplotypes were exhibited with haplotype diversity value about 0.942. The nucleotide diversity for COI sequences of B. dorsalis populations was relatively low ranging from 0.00775–0.00964. The phylogenetic tree from the mtCOI sequences inferred using the Maximum Likelihood method indicated monophyly with strong support of B. dorsalis. Nevertheless, it was suggested that various collection sites in each region throughout Thailand should be initially conducted. Additionally, the further study should be accompanied by using the other sibling species of B. dorsalis complex and analyzing DNA sequences from both mitochondrial and nuclear genes in order to obtain distinctly information in terms of phylogeography.