Extraction of Polysaccharides from Hericium erinaceus by Ultrasound Combined with Heat Treatment and Effect on the Growth of Lactic Acid Bacteria

Abstract

The objective of this research was to study the effect of ultrasound combined with heat treatment on the extraction of polysaccharides from the Hericium erinaceus and to study the growth promotion effect of the products on lactic acid bacteria. Firstly, the Hericium erinaceus in powder form was subjected to ultrasound process at 100, 200, and 300 W under 50, 60, and 70 degree celsius for 20, 40, and 60 minutes. Then the crude extracts were analyzed for reducing sugar and total sugar. The result showed that the maximum total sugar and reducing sugar content were exhibited at 25.9 % (w/w) and 11.4 %( w/w), respectively, at an ultrasound power of 200 W at 70 degree celsius for 60 minutes. After purified by de-starch and deprotein followed by dialysis, the polysaccharide was analyzed for growth promotion of 4 strains of Lactobacillus spp. i.e.  L. bulgaricus, L. brevis, L. johnsonii, and L. plantarum. The results showed that the polysaccharide from Hericium erinaceus could significantly promote the growth of L. bulgaricus and L. brevis.