The Study of Optimum Growth Condition in Some Ectomycorrhizal Mushroom In Vitro

Abstract

Phlebopus portentosus and Astraeus odoratus were ectomycorrhiza fungi found in natural forests. They became the most popular consumer and found expensive wild edible mushrooms since they were rarely grown only on rainy season and were unable to be cultivated normally in the mushroom green house. For this reason, in this study of suitable growth conditions of P. portentosus and A. odoratus were cultivated on 9 cultures medium including Potato Dextose Agar (PDA), Potato Glucose Agar (PGA), Modified Potato Glucose Agar (mPGA), Malt Agar (MA), Malt Extract Agar (MEA), Modified Melin-Norkran Medium (MMN), Oat Agar (OA), Yeast Extract Agar (YEA) and Potato Carrot Agar (PCA), incubated at 20, 25, 30 and 35 °C.  They were exhibited significantly different (P≤0.05) between P. portentosus and A. odoratus with the highest growth rate mycelia of 0.220±0.05 and 0.298±0.06 cm./day, respectively on PCA medium, at 25 °C. Thereafter, they were cultured on the liquid medium of PCB, PGB and MMN broth in different pH values (pH 5, 6, 7, 8, 9), they were found continuosly growing with thick rhizomorph mycelium. Moreover, the best growth rate of P. portentosus was found in PCB while that rate of A. odoratus found in PGB, at pH 6 with the biomass content of 0.514±0.011 g /100 ml and 0.226±0.003 g /100 ml, respectively. This research presents the first important steps in increasing of ectomycorrhizal mushrooms for inoculatation in host plants and this will lead to the number of future success for social and communities in forest plantations.