Inheritance of antioxidant traits of the brown rice in F2 population from the cross of “RD41” × “Riceberry”
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Abstract
A study on inheritance of antioxidant traits in brown rice was an important basic genetic information for estimating the possibility of rice selection with high antioxidant traits in the progeny population of “RD41” × “Riceberry. This population was generated and planted at Pathumthani Rice Research Center between August 2016 and November 2017. The objective was to study the inheritance of antioxidant traits of brown rice in the F2 population derived from RD41 (P1) × Riceberry (P2). The brown rice of P1, P2, F1, and F2 were extracted with 1% (v/v) hydrochloric acid in methanol. Then, the content of the antioxidant traits including total phenolic content (TPC), total flavonoid content (TFC) and total anthocyanin content (TAC), and antioxidant activity including ABTS radical scavenging assay (ABTS), and Ferric reducing antioxidant power (FRAP) were analyzed. The results found that the colors of rice pericarp were regulated by two recessive genes, and the frequency distributions of F2antioxidant traits were continuous and non-normal distribution. Furthermore, the distribution showed transgressive segregation. The high positive correlations between all antioxidant traits were detected (r=0.910-0.973, p=0.01). The genotypic coefficient of variation (GCV), phenotypic coefficient of variation (PCV), heritability (H2), and percentage of genetic advance (%GA) exhibited the high value and ranged from 40.08-117.62, 44.13-124.35, 0.82-0.94 and 75.16-229.74, respectively and the difference between GCV and PCV were low value and ranged from 1.57-6.73. The results suggested that the antioxidant traits in this population were expressed by quantitative inheritance of polygene, regulated by recessive genes, and indicated to be influenced by genes rather than the environment. Consequently, the possibility of selection in rice cultivar for the antioxidant traits from this population was successful.
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