Effects of carbon and nitrogen sources on fruiting body formation and cordycepin production of Cordyceps militaris (L.) Link

บทคัดย่อ

Cordyceps militaris (L.) Link is an entomopathogenic fungus. It has been accumulated the bioactive compounds including cordycepin and adenosine that reduced blood cholesterol and provide improved anti-cancer, and immune support. The objectives of this study were to establish an effective medium for fruiting body formation of C. militaris and to evaluate cordycepin and adenosine contents. Each medium contained different types of carbon sources (sucrose, glucose) and nitrogen sources (yeast extract, peptone). For the BH strain, the highest length of fruiting bodies (4.61±0.45 cm), fresh weight (35.37 ± 1.16 g/bottle) dry weight (11.88±0.29 g/bottle) and cordycepin contents (6.92±0.09 mg/g) were observed on T6 medium supplemented with 20 g/l sucrose and 20 g/l yeast extract and the highest adenosine contents (0.10±0.02 mg/g) were found in T2 medium supplemented with 10 g/l sucrose and 10 g/l yeast extract. For the DA strain, the highest length of fruiting bodies (4.26±0.08 cm), fresh weight (31.89±0.25 g/bottle) dry weight (10.02 ± 0.15 g) and cordycepin contents (3.95±0.05 mg/g) were obtained from T6 medium supplemented with 20 g/l sucrose and 20 g/l yeast extract and the highest adenosine contents (0.72±0.00 mg/g) was achieved on T5 medium supplemented with 20 g/l glucose and 20 g/l peptone.