Polymorphisms of ADRB3, FABP3, IGF2, and SCD genes in Kradon Pig
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Apr 5, 2021
Keywords:
ADRB3 gene FABP3 gene IGF2 gene SCD gene Kradon Pig
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Abstract
Meat of native pig offers better taste than the commercial ones. Thus, raising native pig has become popular and desired by consumers. However, the pigs have a low growth rate and fatty carcass. Intramuscular fat of the pigs and its compositions plays an important role in meat quality and carcass percentage. The objective of this research was to study the genotypes of ADRB3, FABP3, IGF2, and SCD genes using PCR-RFLP. The analysis of genotypes frequency, allele frequency, and Hardy-Weinberg equilibrium (HWE) was tested with the Chi-square test (c2-test) in Kradon pigs. The results showed polymorphic had three genotypes in the ADRB3, FABP3, IGF2 genes except the SCD gene was found only one genotype (CT). Analysis for the genotype frequency of the ADRB3 gene indicated that the genotype frequency of the AG was 0.481, which was higher than the GG and AA (0.319 and 0.198, respectively). For the FABP3 gene, the frequency of genotypes of HH, Hh, and hh were 0.380, 0.391, and 0.228, respectively. For the IGF2 gene the GG and CG genotypes were higher than the CC genotypes, and Kradon pigs have G allele of IGF2 as a basal allele. The HWE test showed that the genes of ADRB3, FABP3, and IGF2 were in equilibrium according to the rules of Hardy- Weinberg equilibrium. Therefore, it is possible to use genotypes of the ADRB3, FABP3, and IGF2 genes in this study to find relationships with the carcass quality and meat quality in pigs to develop the genetic markers for the selection and genetic improvement of Kradon pigs in the future.
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Buasook, T. ., Ampaporn, K. ., & Suwannasing, R. . (2021). Polymorphisms of ADRB3, FABP3, IGF2, and SCD genes in Kradon Pig. Khon Kaen Agriculture Journal, 49(2), 458–468. retrieved from https://li01.tci-thaijo.org/index.php/agkasetkaj/article/view/249615
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บทความวิจัย (research article)
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References
เกศรา อำพาภรณ์, พิลาสลักษณ์ ปานประเสริฐ, ชเวง สารคล่อง และมนต์ชัย ดวงจินดา. 2561. ผลของยีน IGF-II ต่อลักษณะการเจริญเติบโตและคุณภาพซากในสุกรพันธุ์กระโดน. แก่นเกษตร. 46: 533-542.
จตุพร คูณแก้ว. 2551. การศึกษาลักษณะซากและคุณภาพเนื้อของสุกรพื้นเมืองไทย. วิทยานิพนธ์ตามหลักสูตรปริญญาวิทยาศาสตรมหาบัณฑิต สาขาเทคโนโลยีการผลิตสัตว์ มหาวิทยาลัยเทคโนโลยีสุรนารี, นครราชสีมา.
จรัญ จันทลักขณา. 2524. การปรับปรุงพันธุ์หมูเมืองไทยในอนาคต. สุกรสาส์น. 7: 27-45.
จรัญ จันทลักขณา. 2526. ปศุสัตว์สำหรับท้องถิ่น. กรุงเทพมหานคร: สำนักพิมพ์จุฬาลงกรณ์มหาวิทยาลัย, กรุงเทพฯ.
ธวัชชัย แถวถาทำ และประภาส มหินชัย. 2560. การวิเคราะห์ความสัมพันธ์ของยีน IGF2 ต่อสมรรถนะการผลิตของสุกร. วารสารวิชาการ สำนักพัฒนาพันธุ์สัตว์, กรมปศุสัตว์ กระทรวงเกษตรและสหกรณ์, กรุงเทพฯ..
แพรว เที่ยงพิมล, ธนาทิพย์ สุวรรณโสภี, ศุภมิตร เมฆฉาย และศกร คุณวุฒิฤทธิรณ. 2552. ความสัมพันธ์ของความแตกต่างทางพันธุกรรมของยีน Insulin-like growth factor II (IGF-II) ต่อลักษณะการเจริญเติบโตและขนาดร่างกายในประชากรสุกรเชิงการค้าแห่งหนึ่ง. แก่นเกษตร. 37: 319-330.
วินัย ประลมพ์กาญจน์ และผกาพรรณ สกุลมั่น. 2543. เอกสารการสอนชุดวิชาการปรับปรุงพันธุ์ และการสืบพันธุ์สัตว์ หน่วยที่ 1-7. มหาวิทยาลัยสุโขทัยธรรมาธิราช, นนทบุรี.
Botstein, D., R. L. White, M. Skolnick, and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics. 32: 314-331.
Chikun, K., A. Horiuchi, H. Ide, M. Shibata, T. Hayashi, I. Nakajima, M. Oe, and S. Muroya. 2008. Nucleotide sequence polymorphisms of beta1, beta2, and beta3-adrenergic receptor genes on Jinhua, Meishan, Duroc and Landrace pigs. Animal Science Journal. 79: 665-672.
Cieslak, J., J. Nowacka-Woszuk, M. Bartz, H. Fijak-Nowak, M. Grzes, M. Szydlowski, and M. Switonski. 2009. Association studies on the porcine RETN, UCP1, UCP3 and ADRB3 genes polymorphism with fatness traits. Meat Science. 83: 551-554.
Dekkers, J. C. M. 2004. Commercial Application of Marker- and Gene-assisted Selection in Livestock: Strategies and Lessons. Journal of Animal Science. 82: 313-328.
Enoch, H.G., A. Catala, and P. Strittmatter. 1976. Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid. Journal of Biological Chemistry. 251: 5095-5103.
Estany, J., R. Ros-Freixedes, M. Tor, and R. N. Pena. 2014. A functional variant in the stearoyl-CoA desaturase gene promoter enhances fatty acid desaturation in pork. PloS One. 9: e86177.
Falcorner, D. S. and T. F. C. Mackey. 1996. Introduction to Quantitative Genetics. 4th Edition. Pearson Education, New York.
Fortin, A., W. M. Robertson, and A. K. W. Tong. 2005. The eating quality of Canadian pork and its relationship with intramuscular fat. Meat Science. 69: 297-305.
Goodwin, W., A. Linacre, and S. Hadi. 2007. An Introduction to Forensic Genetics. John Wiley & Sons Ltd., Chicheste.
Henriquez-Rodriguez, E., M. Tor, R. N. Pena, and J. Estany. 2015. A polymorphism in the stearoyl-CoA desaturase gene promoter increases monounsaturated fatty acid content in dry-cured ham. Meat Science. 106: 38-43.
Klomtong, P., K. Chaweewan, Y. Phasuk, and M. Duangjinda. 2015. MC1R, KIT, IGF2, and NR6A1 as markers for genetic differentiation in Thai native, wild boars, and Duroc and Chinese Meishan pigs. Genetics and molecular research. 14: 12723-12732.
Knoll, A., L. Putnova, J. Dvorak, and S. Cepica. 2000. A NciI PCR-RFLP within intron 2 of the porcine insulin-like growth factor 2 (IGF2) gene. Animal Genetics. 31: 150-151.
Lee, S. H., Y. M. Choi, J. H. Choe, J. M. Kim, K. C. Hong, H. C. Park, and B. C. Kim. 2010. Association between polymorphisms of the heart fatty acid binding protein gene and intramuscular fat content, fatty acid composition, and meat quality in Berkshire breed. Meat Science. 86: 794-800.
Lim, K. S., J. M., Kim, E. A. Lee, J. H. Choe, and K. C. Hong. 2015. A Candidate Single Nucleotide Polymorphism in the 3’ Untranslated Region of Stearoyl-CoA Desaturase Gene for Fatness Quality and the Gene Expression in Berkshire Pigs. Asian Australasian Journal of Animal Sciences. 28: 151-157.
Liu, G. L., S. W. Jiang, Y. Z. Xiong, R. Zheng, and Y. C. Qu. 2003. Association of PCR-RFLP polymorphisms of IGF2 gene with fat deposition related traits in pig resource family. Acta Genetica Sinica. 30: 1107-1112.
Maharani, D., H. B. Park, J. B. Lee, C. K. Yoo, H. T. Lim, S. H. Han, S. S. Lee, M. S. Ko, I. C. Cho, and J. H. Lee. 2013. Association of the gene encoding stearoyl-CoA desaturase (SCD) with fatty acid composition in an intercross population between Landrace and Korean native pigs. Molecular Biology Reports. 40: 73-80.
Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 89: 583-590.
Nowacka-Woszuk, J., I. Szczerbal, H. Fijak-Nowak, and M. Switonski. 2008. Chromosomal localization of 13 candidate genes for human obesity in the pig genome. Journal of Applied Genetics. 49: 373-377.
Ranjbari, M., A. Hashemi, K. Mardani, and R. Darvishzadeh. 2012. Allelic polymorphism of Makoei sheep calpastatin gene identified by polymerase chain reeaction and single strand conformation polymorphism. Journal of Agricultural Science and Technology. 14: 533-538.
Reardon, W., A. M. Mullen, T. Sweeney, and R. M. Hamill. 2010. Association of polymorphisms in candidate genes with colour, water-holding capacity, and composition traits in bovine M. longissimus and M. semimembranosus. Meat Science. 86: 270-275.
Ren, J., C. Knorr, F. Habermann, R. Fries, L. S. Huang, and B. Brenig. 2003. Assignment of the porcine stearoyl-CoA desaturase (SCD) gene to SSC14q27 by fluorescence in situ hybridization and by hybrid panel mapping. Animal Genetics. 34: 471-473.
Samulin, J., I. Berget, S. Lien, and H. Sundvold. 2008. Differential gene expression of fatty acid binding proteins during porcine adipogenesis. Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology. 151: 147-152.
Tanaka, K., Y. Iwaki, T. Takizawa, M. Murakami, H. Mannen, Y. Maeda, Y. Kurosawa, V. B. Dand, L. Chhum Phith, B. Bouahom, Y. Yamamoto, T. Daing, and T. Namikawa. 2007. The novel polymorphism of the beta 3-adrenergic receptor gene and its distribution in domestic pigs and wild boars in Asia. Animal Science Journal. 78: 243-250.
Tramontana, S., M. Bionaz, A. Sharma, D. E. Graugnard, E. A. Cutler, and P. Ajmone-Marsan. 2008. Internal controls for quantitative polymerase chain reaction of swine mammary glands during pregnancy and lactation. Journal of Dairy Science. 91: 3057-3066.
Uemoto, Y., H. Nakano, T. Kikuchi, S. Sato, M. Ishida, T. Shibata, H. Kadowaki, E. Kobayashi, and K. Suzuki. 2012. Fine mapping of porcine SSC14 QTL and SCD gene effects on fatty acid composition and melting point of fat in a Duroc purebred population. Animal Genetics. 43: 225-228.
Urban, T., A. Knoll, S. Boleslavova, A. Schmidtova, H. Sulcerova, V. Rozikova, and M. Juzl. 2016. Polymorphism in the gene FABP3 and its association with indicators of meat quality in pigs. Acta fytotechn zootechn. 19: 171-173.
Van Laere, A. S., M. Nguyen, M. Braunschweig, C. Nezer, C. Collette, L. Moreau, A. L. Archibald, C. S. Haley, N. Buys, M. Tally, G. Andersson, M. Georges, and L. Andersson. 2003. A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature. 425: 832-836.
Vasupen, K. 2007. Nutritional studies in native, Thai Kadon pigs. Ph.D. Thesis. Department of Nutrition Faculty of Veterinary Medicine, Utrecht University. Utrecht, The Netherlands.
Vykoukalova, Z., A. Knoll, J. Dvorak, and S. Cepica. 2006. New SNPs in the IGF2 gene and association between this gene and back fat thickness and lean meat content in Large White pigs. Journal of Animal Breeding and Genetics. 123: 204-207.
Wang, W., W. Xue, X. Zhou, L. Zhang, J. Wu, L. Qu, B. Jin, X. Zhang, F. Ma, and X. Xu. 2013. Effects of candidate genes' polymorphisms on meat quality traits in pigs. Acta Agriculturae Scandinavica, Section A – Animal Science. 62: 120-126.
Xue, W., W. Wang, B. Jin, X. Zhang, and X. Xu. 2015. Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition. Czech Journal of Animal Science. 60: 60-66.
Zhang, S., T. J. Knight, K. J. Stalder, R. N. Goodwin, S. M. Lonergan, and D. C. Beitz. 2009. Effects of breed, sex and halothane genotype on fatty acid composition of triacylglycerols and phospholipids in pork longissimus muscle. Journal of Animal Breeding and Genetics. 126: 259-268.
จตุพร คูณแก้ว. 2551. การศึกษาลักษณะซากและคุณภาพเนื้อของสุกรพื้นเมืองไทย. วิทยานิพนธ์ตามหลักสูตรปริญญาวิทยาศาสตรมหาบัณฑิต สาขาเทคโนโลยีการผลิตสัตว์ มหาวิทยาลัยเทคโนโลยีสุรนารี, นครราชสีมา.
จรัญ จันทลักขณา. 2524. การปรับปรุงพันธุ์หมูเมืองไทยในอนาคต. สุกรสาส์น. 7: 27-45.
จรัญ จันทลักขณา. 2526. ปศุสัตว์สำหรับท้องถิ่น. กรุงเทพมหานคร: สำนักพิมพ์จุฬาลงกรณ์มหาวิทยาลัย, กรุงเทพฯ.
ธวัชชัย แถวถาทำ และประภาส มหินชัย. 2560. การวิเคราะห์ความสัมพันธ์ของยีน IGF2 ต่อสมรรถนะการผลิตของสุกร. วารสารวิชาการ สำนักพัฒนาพันธุ์สัตว์, กรมปศุสัตว์ กระทรวงเกษตรและสหกรณ์, กรุงเทพฯ..
แพรว เที่ยงพิมล, ธนาทิพย์ สุวรรณโสภี, ศุภมิตร เมฆฉาย และศกร คุณวุฒิฤทธิรณ. 2552. ความสัมพันธ์ของความแตกต่างทางพันธุกรรมของยีน Insulin-like growth factor II (IGF-II) ต่อลักษณะการเจริญเติบโตและขนาดร่างกายในประชากรสุกรเชิงการค้าแห่งหนึ่ง. แก่นเกษตร. 37: 319-330.
วินัย ประลมพ์กาญจน์ และผกาพรรณ สกุลมั่น. 2543. เอกสารการสอนชุดวิชาการปรับปรุงพันธุ์ และการสืบพันธุ์สัตว์ หน่วยที่ 1-7. มหาวิทยาลัยสุโขทัยธรรมาธิราช, นนทบุรี.
Botstein, D., R. L. White, M. Skolnick, and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics. 32: 314-331.
Chikun, K., A. Horiuchi, H. Ide, M. Shibata, T. Hayashi, I. Nakajima, M. Oe, and S. Muroya. 2008. Nucleotide sequence polymorphisms of beta1, beta2, and beta3-adrenergic receptor genes on Jinhua, Meishan, Duroc and Landrace pigs. Animal Science Journal. 79: 665-672.
Cieslak, J., J. Nowacka-Woszuk, M. Bartz, H. Fijak-Nowak, M. Grzes, M. Szydlowski, and M. Switonski. 2009. Association studies on the porcine RETN, UCP1, UCP3 and ADRB3 genes polymorphism with fatness traits. Meat Science. 83: 551-554.
Dekkers, J. C. M. 2004. Commercial Application of Marker- and Gene-assisted Selection in Livestock: Strategies and Lessons. Journal of Animal Science. 82: 313-328.
Enoch, H.G., A. Catala, and P. Strittmatter. 1976. Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid. Journal of Biological Chemistry. 251: 5095-5103.
Estany, J., R. Ros-Freixedes, M. Tor, and R. N. Pena. 2014. A functional variant in the stearoyl-CoA desaturase gene promoter enhances fatty acid desaturation in pork. PloS One. 9: e86177.
Falcorner, D. S. and T. F. C. Mackey. 1996. Introduction to Quantitative Genetics. 4th Edition. Pearson Education, New York.
Fortin, A., W. M. Robertson, and A. K. W. Tong. 2005. The eating quality of Canadian pork and its relationship with intramuscular fat. Meat Science. 69: 297-305.
Goodwin, W., A. Linacre, and S. Hadi. 2007. An Introduction to Forensic Genetics. John Wiley & Sons Ltd., Chicheste.
Henriquez-Rodriguez, E., M. Tor, R. N. Pena, and J. Estany. 2015. A polymorphism in the stearoyl-CoA desaturase gene promoter increases monounsaturated fatty acid content in dry-cured ham. Meat Science. 106: 38-43.
Klomtong, P., K. Chaweewan, Y. Phasuk, and M. Duangjinda. 2015. MC1R, KIT, IGF2, and NR6A1 as markers for genetic differentiation in Thai native, wild boars, and Duroc and Chinese Meishan pigs. Genetics and molecular research. 14: 12723-12732.
Knoll, A., L. Putnova, J. Dvorak, and S. Cepica. 2000. A NciI PCR-RFLP within intron 2 of the porcine insulin-like growth factor 2 (IGF2) gene. Animal Genetics. 31: 150-151.
Lee, S. H., Y. M. Choi, J. H. Choe, J. M. Kim, K. C. Hong, H. C. Park, and B. C. Kim. 2010. Association between polymorphisms of the heart fatty acid binding protein gene and intramuscular fat content, fatty acid composition, and meat quality in Berkshire breed. Meat Science. 86: 794-800.
Lim, K. S., J. M., Kim, E. A. Lee, J. H. Choe, and K. C. Hong. 2015. A Candidate Single Nucleotide Polymorphism in the 3’ Untranslated Region of Stearoyl-CoA Desaturase Gene for Fatness Quality and the Gene Expression in Berkshire Pigs. Asian Australasian Journal of Animal Sciences. 28: 151-157.
Liu, G. L., S. W. Jiang, Y. Z. Xiong, R. Zheng, and Y. C. Qu. 2003. Association of PCR-RFLP polymorphisms of IGF2 gene with fat deposition related traits in pig resource family. Acta Genetica Sinica. 30: 1107-1112.
Maharani, D., H. B. Park, J. B. Lee, C. K. Yoo, H. T. Lim, S. H. Han, S. S. Lee, M. S. Ko, I. C. Cho, and J. H. Lee. 2013. Association of the gene encoding stearoyl-CoA desaturase (SCD) with fatty acid composition in an intercross population between Landrace and Korean native pigs. Molecular Biology Reports. 40: 73-80.
Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 89: 583-590.
Nowacka-Woszuk, J., I. Szczerbal, H. Fijak-Nowak, and M. Switonski. 2008. Chromosomal localization of 13 candidate genes for human obesity in the pig genome. Journal of Applied Genetics. 49: 373-377.
Ranjbari, M., A. Hashemi, K. Mardani, and R. Darvishzadeh. 2012. Allelic polymorphism of Makoei sheep calpastatin gene identified by polymerase chain reeaction and single strand conformation polymorphism. Journal of Agricultural Science and Technology. 14: 533-538.
Reardon, W., A. M. Mullen, T. Sweeney, and R. M. Hamill. 2010. Association of polymorphisms in candidate genes with colour, water-holding capacity, and composition traits in bovine M. longissimus and M. semimembranosus. Meat Science. 86: 270-275.
Ren, J., C. Knorr, F. Habermann, R. Fries, L. S. Huang, and B. Brenig. 2003. Assignment of the porcine stearoyl-CoA desaturase (SCD) gene to SSC14q27 by fluorescence in situ hybridization and by hybrid panel mapping. Animal Genetics. 34: 471-473.
Samulin, J., I. Berget, S. Lien, and H. Sundvold. 2008. Differential gene expression of fatty acid binding proteins during porcine adipogenesis. Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology. 151: 147-152.
Tanaka, K., Y. Iwaki, T. Takizawa, M. Murakami, H. Mannen, Y. Maeda, Y. Kurosawa, V. B. Dand, L. Chhum Phith, B. Bouahom, Y. Yamamoto, T. Daing, and T. Namikawa. 2007. The novel polymorphism of the beta 3-adrenergic receptor gene and its distribution in domestic pigs and wild boars in Asia. Animal Science Journal. 78: 243-250.
Tramontana, S., M. Bionaz, A. Sharma, D. E. Graugnard, E. A. Cutler, and P. Ajmone-Marsan. 2008. Internal controls for quantitative polymerase chain reaction of swine mammary glands during pregnancy and lactation. Journal of Dairy Science. 91: 3057-3066.
Uemoto, Y., H. Nakano, T. Kikuchi, S. Sato, M. Ishida, T. Shibata, H. Kadowaki, E. Kobayashi, and K. Suzuki. 2012. Fine mapping of porcine SSC14 QTL and SCD gene effects on fatty acid composition and melting point of fat in a Duroc purebred population. Animal Genetics. 43: 225-228.
Urban, T., A. Knoll, S. Boleslavova, A. Schmidtova, H. Sulcerova, V. Rozikova, and M. Juzl. 2016. Polymorphism in the gene FABP3 and its association with indicators of meat quality in pigs. Acta fytotechn zootechn. 19: 171-173.
Van Laere, A. S., M. Nguyen, M. Braunschweig, C. Nezer, C. Collette, L. Moreau, A. L. Archibald, C. S. Haley, N. Buys, M. Tally, G. Andersson, M. Georges, and L. Andersson. 2003. A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature. 425: 832-836.
Vasupen, K. 2007. Nutritional studies in native, Thai Kadon pigs. Ph.D. Thesis. Department of Nutrition Faculty of Veterinary Medicine, Utrecht University. Utrecht, The Netherlands.
Vykoukalova, Z., A. Knoll, J. Dvorak, and S. Cepica. 2006. New SNPs in the IGF2 gene and association between this gene and back fat thickness and lean meat content in Large White pigs. Journal of Animal Breeding and Genetics. 123: 204-207.
Wang, W., W. Xue, X. Zhou, L. Zhang, J. Wu, L. Qu, B. Jin, X. Zhang, F. Ma, and X. Xu. 2013. Effects of candidate genes' polymorphisms on meat quality traits in pigs. Acta Agriculturae Scandinavica, Section A – Animal Science. 62: 120-126.
Xue, W., W. Wang, B. Jin, X. Zhang, and X. Xu. 2015. Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition. Czech Journal of Animal Science. 60: 60-66.
Zhang, S., T. J. Knight, K. J. Stalder, R. N. Goodwin, S. M. Lonergan, and D. C. Beitz. 2009. Effects of breed, sex and halothane genotype on fatty acid composition of triacylglycerols and phospholipids in pork longissimus muscle. Journal of Animal Breeding and Genetics. 126: 259-268.