Association of chlorophyll content and spad chlorophyll in diverse sweet sorghum cultivars under different environments
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Sweet sorghum (Sorghum bicolor (L.) Moench) can be an effective crop for bio-ethanol production due to its adaptability and high sugar yields. Like many other crops, chlorophyll content is related to high productivity and can be measured directly and indirectly. However, literature is lacking concerning non-destructive measurement of chlorophyll in sweet sorghum. The objectives of this study were to determine the most effective technique and best plant age for estimation of chlorophyll content by SPAD chlorophyll meter readings (SCMR). Chlorophyll content and SCMR were taken at 10 day intervals between 40 and 100 days after planting (DAP) at the Field Crops Research Station, Khon Kaen University (KKU) and at the National Corn and Sorghum Research Center, Nakhon Rachsima (NCSRC). SCMR and chlorophyll content were significantly correlated for all test dates at KKU (r = 0.49 to 0.64) but only moderately correlated at 40 DAP (r = 0.48), 90 DAP (r = 0.33) and 100 DAP (r = 0.32) for the NCSRC location. SCMR values had higher correlation between locations and across sample times than leaf chlorophyll content. Differences among entries were significant at all plant ages for SCMR at KKU but for only 70, 80, 90, and 100 DAP for NCSRC. Differences among genotypes were also observed for chlorophyll content at both locations for 80, 90 and 100 DAP. The SPAD chlorophyll meter is a useful tool for indirectly measuring leaf chlorophyll content in sweet sorghum, and has several advantages over direct measurement, including reduced labor and better reproducibility.
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