การประเมินคุณสมบัติทางพฤษเคมีของหญ้าขัดมอญใบยาวและผลการเสริมในอาหารต่อความเข้มข้นพิวรีน อนุพันธ์พิวรีน และกรดยูริกในพลาสมาของไก่โคราช
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ก.พ. 25, 2020
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หญ้าขัดมอญใบยาว แซนทีนออกซิเดส ฟลาโวนอยด์ อัลโลพูรินอล กรดยูริก
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บทคัดย่อ
การทดลองนี้มีวัตถุประสงค์เพื่อประเมินคุณสมบัติทางพฤษเคมีของหญ้าขัดมอญใบยาวและผลการเสริมในอาหารต่อความเข้มข้นสารพิวรีน อนุพันธ์พิวรีน และกรดยูริกในพลาสมาของไก่โคราช โดยใช้ไก่โคราชคละเพศที่อายุ 3 สัปดาห์ จำนวน 40 ตัว แบ่งเป็น 5 กลุ่มทดลอง ๆ ละ 8 ซ้ำ ตามแผนการทดลองแบบสุ่มสมบูรณ์ (CRD) โดยเลี้ยงไก่แต่ละตัวในกรงขังเดี่ยว เป็นระยะเวลา 10 วัน อาหารทดลองแบ่งออกเป็น 5 กลุ่ม 1) กลุ่มควบคุมลบ 2) กลุ่มควบคุมบวก (อัลโลพูรินอล 25 มก./กก.อาหาร) 3) หญ้าขัดมอญใบยาว 0.28% (ฟลาโวนอยด์ 25 มก./กก.อาหาร) 4) หญ้าขัดมอญใบยาว 0.56% (ฟลาโวนอยด์ 50 มก./กก.อาหาร) และ5) หญ้าขัดมอญใบยาว 1.12% (ฟลาโวนอยด์ 100 มก./กก.อาหาร) ผลการศึกษาพบว่าหญ้าขัดมอญใบยาวมีสารประกอบฟลาโวนอยด์แบบหยาบทั้งหมด 8,930 มก./กก. เคมเฟอรอล 311 มก./กก. ค่าความเข้มข้นที่สารสกัดสามารถยับยั้งกิจกรรมของเอนไซม์แซนทีนออกซิเดสได้ 50% เท่ากับ 464.29 มคก. สารสกัด/มล. การเสริมหญ้าขัดมอญใบยาวในอาหารไก่โคราช 0.28% และ 0.28-0.56% สามารถลดความเข้มข้นสารไฮโพแซนทีนและกรดยูริกในพลาสมาลงได้ตามลำดับ (P<0.05) อย่างไรก็ตามเป็นที่น่าสนใจว่าการเสริมอัลโลพูรินอล มีผลทำให้เกิดสารพิวรีน อนุพันธ์พิวรีน และกรดยูริกในพลาสมาสูงที่สุด จากการทดลองครั้งนี้สรุปได้ว่า หญ้าขัดมอญมีคุณสมบัติในการลดการทำงานของเอนไซม์แซนทีนออกซิเดสได้ โดยการเสริมในอาหารไก่โคราชในระดับที่เหมาะสมสามารถลดการผลิตพิวรีน อนุพันธ์พิวรีน และกรดยูริกได้
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References
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Konate, K., A. Souza, A. Y. Coulibaly, N. T. R. Meda, M. Kiendrebeogo, A. Lamien-Meda, J. Millogo-Rasolodimby, M. L. amidaiand, and O. G. Nacoulma. 2010. In vitro antioxidant, lipoxygenase and xanthine oxidase inhibitory activities of fractions from Cienfuegosia digitata Cav., Sida alba L. and Sida acuta Burm.f.(Malvaceae). PJBS. 13(23): 1092-1098
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Lumeij, J.T., E.P.M. Sprang and P.T. Redig. 1998. Further studies on allopurinol-induced hyperuricaemia and visceral gout in red-tailed hawks (Buteo jamicensis). Avian Pathol. 27(4): 390–393
Maliwan, P. 2016. Energy and protein requirements of Korat chickens from 0 to 12 weeks of age. Ph. D. Thesis. Suranaree University of Technology, Nakhon Ratchasima.
Mandell, B. F. 2002. Hyperuricemia and gout: a reign of complacency. Cleve. Clin. J. Med. 69(8): 583–592
Nacoulon, O. G. 1996. Medicinal plants and their traditional uses in Burkina Faso. Ph. D. Thesis. University of Ouagadougou, Ouagadougou
Nagao, A., M. Seki, and H. Kobayachi. 1999. Inhibition of xanthine oxidase by flavonoids. Biosci. Biotechnol. Biochem. 63(10): 1787-1790
Niu, Y., X. L. Wan, X. H. Zhang, L. G. Zhao, J. T. He, J. F. Zhang, L. L. Zhang, and T. Wang. 2016. Effect of supplemental fermented Ginkgo biloba leaves at different levels on growth performance, meat quality, and antioxidant status of breast and thigh muscles in broiler chickens. Poul. Sci. 96(4): 869-877
Ohno, I., Y. Ishida, T. Hosoya, M. Kobayashi, and O. Sakai. 1990. Allopurinol induced aplastic anemia in a patient with chronic renal failure. Ryumachi. 30(4): 281–286
Okafuji, K., and K. Shinohara. 1990. Aplastic anemia probably induced by allopurinol in a patient with renal insufficiency. Rinsho Ketsueki. 31(1): 85–88
Poffers, J., J.T. Lumeij, E.P.M. Timmermans-Sprang and P.T. Redig. 2002. Further studies on the use of allopurinol to reduce plasma uric acid concentrations in the red-tailed hawk (Buteo jamicensis) hyperuricaemic model. Avian Pathol. 31(6); 567–572
Prakash, A., R. K. Varma, and S. Ghosal. 1981. Alkaloid constituents of Sida acuta, S. humilis, S. rhombifolia and S. spinosa. Planta. Med. 43(2): 384-388
Raimi, M. M., M. Oyekanmi, and B. M. Adegoke. 2014. Proximate, phytochecal and micronutrient composition of Sida acuta. IOSR J. App. Chem. 7(2): 93-98
Saganuwan, A. S, and M. L. Gulumbe. 2006. Evaluation of Sida acuta subspecie acuta leaf and flower combination for antimicrobial activity and phytochemical constituents. Afr. J. Clin. Exp. Microbiol. 2(7): 83-88
Sarawek, S. 2007. Xanthine oxidase inhibition and antioxidant activity of an artichoke leaf extract (cynarascolymus l.) and its compounds. Ph. D. Thesis. University of Florida, Florida
Settle, T., M. D. Carro, E. Falkenstein, W. Radke, and H. Klandrof. 2012. The effects of allopurinol, uric acid, and inosine administration on xanthine oxidoreductase activity and uric acid concentrations in broilers. Poul. Sci. 91(11): 2895–2903
Stirpe, F., and E. Della Corte. 1965. Regulation of xanthine dehydrogenase in chick liver and effect of starvation and of administration of purines and purine nucleosides. Biochem. J. 94(2): 309– 313
Stote, R. M., L. H. Smith, J. W. Dubb, T. P. Moyer, F. Alexander, and J. L. Roth. 1980. Oxypurinol nephrolithiasis in regional enteritis secondary to allopurinol therapy. Ann. Intern. Med. 92(3): 384–385.
Van B. , T. A., and P. Montoro. 2009. Chemical analysis and quality control of Ginkgo biloba leaves, extracts, and phytopharmaceuticals. J. Chromatogr. A. 12(16): 2002–2032
Wang, H., and K. Helliwell. 1998. Determination of flavonols in tea leaves by HPLC with isocratic elution system. Food Res. Int. 34(2-3): 223-227
Yao, L. H., Y. M. Jiang, J. Shi, F. A. Tomas-Barberan, N. Datta, R. Singanusong, and S. S. Chen. 2004. Flavonoids in food and their health benefits. Plant Foods Hum. Nutr. 59(3): 113–122
Asha, A., F. Shameema, and E. C. Baiju. 2018. Phytochemical profiling and antibacterial activity of selected Sida species against common human pathogenic bacteria an in vitro study. JPP. 7(3): 1201-1205
Cao, F. L., X. H. Zhang, W. W. Yu, L. G. Zhao, and T. Wang. 2012. Effect of feeding fermented Ginkgo biloba leaves on growth performance, meat quality, and lipid metabolism in broilers. Poul. Sci. 91(5): 1210–1221
Carro, M. D., E. Falkenstein, W. J. Radke, and H. Klandorf. 2010. Effects of allopurinol on uric acid concentrations, xanthine oxidoreductase activity and oxidative stress in broiler chickens. Comp. Biochem. Physiology. C. T. Pharm. 151(1): 12–17
Chang, W. S., Y. J. Lee, F. J. Lu, and H. C. Chiang. 1993. Inhibitory effects of flavonoids on xanthine oxidase. Anticancer Res. 13(6A): 2165-2170
Cotelle, N., J. L. Bernier, J. P. Catteau, J. Pommery, J. C. Wallet, and E. M. Gaydou. 1996. Antioxidant properties of hydroxyl-flavones. Radic. Biol. Med. 20(1): 35-43
Edeoga, H. O., D. E. Okwu, and B. O. Mbaebie. 2005. Phytochemical constituents of some Nigerian medicinal plants. Afr. J. Biotechnol. 4: 685-688
Ellington, A. 2005. Reduction of purine content in commonly consumed meat products through rinsing and cooking. M. S. Thesis. University of Georgia, Athens
Guoyao, W. 2013. Amino acids biochemistry and nutrition. CRC press, NY.
Halde, U. K. 2011. Genus Sida the plants with ethno medicinal and therapeutic potential. ISSN. 1(5):1-4
Havsteen, B. H. 2002. The biochemistry and medical significance of the flavonoids. Pharmacol. Ther. 96(2-3): 67-202
Iniaghe, O. M., S. O. Malomo, and J. O. Adebayo. 2009. Proximate composition and phytochemical constituents of leaves of some Acalypha species. Pak. J. Nutr. 8(3): 256-258
James, M. P, and H. Russ. 2008. Inhibition studies of bovine xanthine oxidase by luteolin, silibinin, quercetin, and curcumin. J. Nat. Prod. 72(4): 725–731
Jindal, A., P. Kumar, and G. Singh. 2010. Extraction and pharmacological evaluation of Sida acuta Burm.f. Int. J. Green Pharmacy. 6(3): 34-41
Kaneko, K., A. Yasuo, F. Tomoko, I. Katsunori, and Y. Noriko. 2014. Total purine and purine base content of common foodstuffs for facilitating nutritional therapy for gout and hyperuricemia. Biol. Pharm. Bull. 37(5): 709–721
Konate, K., A. Souza, A. Y. Coulibaly, N. T. R. Meda, M. Kiendrebeogo, A. Lamien-Meda, J. Millogo-Rasolodimby, M. L. amidaiand, and O. G. Nacoulma. 2010. In vitro antioxidant, lipoxygenase and xanthine oxidase inhibitory activities of fractions from Cienfuegosia digitata Cav., Sida alba L. and Sida acuta Burm.f.(Malvaceae). PJBS. 13(23): 1092-1098
Lin, W. C., M. T. Lee, S. C. Chang, Y. L. Chang, C. H. Shih, B. Yu, and T. T. Lee. 2016. Effects of mulberry leaves on production performance and the potential modulation of antioxidative status in laying hens. Poult. Sci. 96(5): 1191-1203
Lumeij, J.T., E.P.M. Sprang and P.T. Redig. 1998. Further studies on allopurinol-induced hyperuricaemia and visceral gout in red-tailed hawks (Buteo jamicensis). Avian Pathol. 27(4): 390–393
Maliwan, P. 2016. Energy and protein requirements of Korat chickens from 0 to 12 weeks of age. Ph. D. Thesis. Suranaree University of Technology, Nakhon Ratchasima.
Mandell, B. F. 2002. Hyperuricemia and gout: a reign of complacency. Cleve. Clin. J. Med. 69(8): 583–592
Nacoulon, O. G. 1996. Medicinal plants and their traditional uses in Burkina Faso. Ph. D. Thesis. University of Ouagadougou, Ouagadougou
Nagao, A., M. Seki, and H. Kobayachi. 1999. Inhibition of xanthine oxidase by flavonoids. Biosci. Biotechnol. Biochem. 63(10): 1787-1790
Niu, Y., X. L. Wan, X. H. Zhang, L. G. Zhao, J. T. He, J. F. Zhang, L. L. Zhang, and T. Wang. 2016. Effect of supplemental fermented Ginkgo biloba leaves at different levels on growth performance, meat quality, and antioxidant status of breast and thigh muscles in broiler chickens. Poul. Sci. 96(4): 869-877
Ohno, I., Y. Ishida, T. Hosoya, M. Kobayashi, and O. Sakai. 1990. Allopurinol induced aplastic anemia in a patient with chronic renal failure. Ryumachi. 30(4): 281–286
Okafuji, K., and K. Shinohara. 1990. Aplastic anemia probably induced by allopurinol in a patient with renal insufficiency. Rinsho Ketsueki. 31(1): 85–88
Poffers, J., J.T. Lumeij, E.P.M. Timmermans-Sprang and P.T. Redig. 2002. Further studies on the use of allopurinol to reduce plasma uric acid concentrations in the red-tailed hawk (Buteo jamicensis) hyperuricaemic model. Avian Pathol. 31(6); 567–572
Prakash, A., R. K. Varma, and S. Ghosal. 1981. Alkaloid constituents of Sida acuta, S. humilis, S. rhombifolia and S. spinosa. Planta. Med. 43(2): 384-388
Raimi, M. M., M. Oyekanmi, and B. M. Adegoke. 2014. Proximate, phytochecal and micronutrient composition of Sida acuta. IOSR J. App. Chem. 7(2): 93-98
Saganuwan, A. S, and M. L. Gulumbe. 2006. Evaluation of Sida acuta subspecie acuta leaf and flower combination for antimicrobial activity and phytochemical constituents. Afr. J. Clin. Exp. Microbiol. 2(7): 83-88
Sarawek, S. 2007. Xanthine oxidase inhibition and antioxidant activity of an artichoke leaf extract (cynarascolymus l.) and its compounds. Ph. D. Thesis. University of Florida, Florida
Settle, T., M. D. Carro, E. Falkenstein, W. Radke, and H. Klandrof. 2012. The effects of allopurinol, uric acid, and inosine administration on xanthine oxidoreductase activity and uric acid concentrations in broilers. Poul. Sci. 91(11): 2895–2903
Stirpe, F., and E. Della Corte. 1965. Regulation of xanthine dehydrogenase in chick liver and effect of starvation and of administration of purines and purine nucleosides. Biochem. J. 94(2): 309– 313
Stote, R. M., L. H. Smith, J. W. Dubb, T. P. Moyer, F. Alexander, and J. L. Roth. 1980. Oxypurinol nephrolithiasis in regional enteritis secondary to allopurinol therapy. Ann. Intern. Med. 92(3): 384–385.
Van B. , T. A., and P. Montoro. 2009. Chemical analysis and quality control of Ginkgo biloba leaves, extracts, and phytopharmaceuticals. J. Chromatogr. A. 12(16): 2002–2032
Wang, H., and K. Helliwell. 1998. Determination of flavonols in tea leaves by HPLC with isocratic elution system. Food Res. Int. 34(2-3): 223-227
Yao, L. H., Y. M. Jiang, J. Shi, F. A. Tomas-Barberan, N. Datta, R. Singanusong, and S. S. Chen. 2004. Flavonoids in food and their health benefits. Plant Foods Hum. Nutr. 59(3): 113–122