Seed priming improves germination of fresh and artificially aged seeds of super sweet corn (Zea mays L. saccharata) by the enhancement of antioxidative mechanism and change in carbohydrate metabolism
Article Sidebar
เผยแพร่แล้ว:
ส.ค. 27, 2018
Main Article Content
บทคัดย่อ
Poor stand establishment is one of the major problems in super sweet corn (Zea maysL. saccharata) production due to a rapid loss in seed vigor. In this study, four priming solutions were investigated for improvement of seed germination of fresh- and artificially aged (AA) seeds of two varieties of super sweet corn seed, Hi-brix53 (hybrid) and open pollinated F3 of ATS5 (OP). The seeds were primed for 12 hours either with distilled water (H2O), 0.33% wood vinegar (WV), 0.59% NaCl or 1.12% CaCl2, to explore theirs effect on the improvement of germination, including physiological and biochemical changes. The results showed that percentage of germination was significantly improved in both fresh and AA seeds of both corn varieties after priming, with the WV treatment. The improvement was associated with the reduction of malondialdehyde (MDA) content in fresh hybrid, AA hybrid and fresh OP seeds. In fresh and AA seeds of hybrid variety, the MDA reductions were associated with the increases of activities of antioxidant enzymes, ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD), which APX was distinguished. However, carbohydrate hydrolysis, determined by invertase and amylase activities, was significantly suppressed in primed fresh hybrid, but not in primed AA seed.In fresh and AA seeds of OP variety, the improvement was not associated with the increases of activities of antioxidant enzymes, but probably involved with carbohydrate hydrolysis.
Article Details
How to Cite
บท
บทความวิจัย (research article)
References
Aebi, H. 1984. Catalase in vitro. Methods in Enzymology 105: 121–126.
Amjad, M., K. Ziaf, Q. Iqbal, I. Ahmad, M. A. Riaz, and Z. A. Saqib. 2007. Effect of seed priming on seed vigour and salt tolerance in hot pepper. Pak. J. Agri. Sci. 44: 408–414.
Andreoli, C., and A. A. Khan. 2000. Integration of physiological, chemical and biological seed treatments to improve stand establishment and yield of vegetables. Acta Hortic. 533: 31–38.
Bailly, C., A. Benamar, F. Corbineau, and D. Come. 1998. Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds. Physiol. Plant 104: 646–652.
Beauchamp, C., and I. Fridovich. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276–287.
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Brecht, J. K., S.A. Sargent, R.C. Hochmuth, and R.S. Tervola. 1990. Postharvest quality of supersweet (sh2) sweet corn cultivars. Proc. Fla. State Hort. Soc. 103: 283–288.
Cao, D., J. Hu, X. Huang, X. Wang, Y.Guan, and Z. Wang. 2008. Relationships between changes of kernel nutritive components and seed vigor during development stages of F1 seeds of sh2 sweet corn. J. Zhejiang Univ. Sci. B 9: 964–968.
Chaplin, M. F. 1986. Monosacharides In: M.F.C. Kennedy (ed.) Carbohydrate Analysis, a Practical Approach. Oxford, Washington DC: IRL Press, pp. 1–36.
Delouche, J. C., and C. C. Baskin. 1973. Accelerated aging techniques for predicting the relative storability of seed lots. Seed Sci. Technol. 1: 427–452.
Demiral, T., and I. Turkan. 2005. Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environ. Exp. Bot. 53: 247–257.
Don, R., ISTA Germination Committee. 2006. ISTA Handbook on Seedling Evaluation. Bassersdorf, Switzerland: International Seed Testing Association.
Esterbauer, H, and K. H. Cheeseman. 1990. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 186: 407–421.
Farooq, M., S. M. A. Barsa, and A. Wahid. 2006. Priming of field-sown rice seed enhances germination, seedling establishment, allometry and yield. Plant Growth Regul. 49: 285–294.
Flematti, G. R., E. L. Ghisalberti, K. W. Dixon, and R.D. Trengove. 2004. A compound from smoke that promotes seed germination. Sci. 305: 977.
George, D. L., M. L. Gupta, D. Tay, and I. G. M.A Parwata. 2003. Influence of planting date, method of handling and seed size on super sweet corn seed quality. Seed Sci. Technol. 31: 351–366.
Gholami, M., F. Mokhtarian, and B. Baninasab. 2015. Seed halopriming improves the germination performance of black seed (Nigella sativa) under salinity stress conditions. J. Crop. Sci. Biotechnol. 18: 21–26.
Goel A, A. K. Goel, and I. S. Sheoran. 2003. Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. J. Plant Physiol. 160: 1093–1100.
Góth, L. 1991. A simple method for determination of serum catalase activity and revision of reference range. Clin. Chim. Acta. 196: 143-152.
Guan, Y., J. Hu, X. Wang, and C. Shao. 2009. Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. J. Zhejiang Univ. Sci. B 10: 427–433.
Harris, D., A. Joshi, P. A. Khan, P. Gothkar, and P. S. Sodhi. 1999. On-farm seed priming in semi-arid agriculture: development and evaluation in maize, rice and chickpea in India using participatory methods. Exp. Agr. 35: 15–29.
Hartz, T. K., and J. Caprile. 1995. Germination of sh2 sweet corn following seed disinfestation, solid-matrix priming, and microbial seed treatment. HortSci. 30: 1400–1402.
Hsu, C. C., C. L. Chen, J. J. Chen, and J. M. Sung. 2003. Accelerated aging-enhanced lipid peroxidation in bitter gourd seeds and effects of priming and hot water soaking treatments. Sci. Hortic. 98: 201–212.
Hussain, S., M. Zheng, F. Khan, A. Khaliq, S. Fahad, S. Peng, J. Huang, K. Cui, and L. Nie. 2015. Benefits of rice seed priming are offset permanently by prolonged storage and the storage conditions. Scientific Reports Jan 29; 5:8101.
Jie, L., L. Gongshe, Q. Dongmei, L. Fangfang, and W. Enhua. 2002. Effect of PEG on germination and active oxygen metabolism in wild rye (Leymus chinensis) seeds. ACTA Pratacult. Sin. 11: 59–64.
Jothityangkoon, D., C. Ruamtakhu, S. Tipparak, S. Wanapat, and A. Polthanee. 2007. Wood vinegar enhances seed germination and seedling development of rice. P. 35–40. In Proc. of the 2nd Int. Conf. on Rice for the Future, Queen Sirikit National Convention Center, Bangkok, Thailand.
Kranner, I., R. P. Beckett, F. V. Minibayeva, and C. E. Seal. 2010. Tansley review: What is stress? Concepts, definitions and applications in seed science. New Phytol. 188: 655-673.
Kulkarni, M. G, S. G. Sparg, S. G., M. E. Light, and J. van Staden. 2006. Stimulation of rice (Oryza sativa L.) seedling vigour by smoke-water and butenolide. J. Agron. Crop Sci. 192: 395–398.
Lertrat, K., and T. Pulam. 2007. Breeding for increased sweetness in sweet corn. Int. J. Plant Breed. 1: 27–30.
Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426–428.
Mukhtar, T., M. Z. Kayani, and M.A. Hussain. 2013. Response of selected cucumber cultivars to Meloidogyne incognita. Crop Prot. 44: 13–17.
Murthy, U. M. N., P. P. Kumar, and W. Q. Sun. 2003. Mechanisms of seed ageing under different storage conditions for Vigna radiata (L.) Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition. J. Exp. Bot. 54: 1057–1067.
Nakano, Y., and K. Asada. 1987. Purification of ascorbate peroxidase in spinach chloroplasts; Its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiol. 28: 131–140.
Patade, V. Y., S. Bhargava, and P. Suprasanna. 2009. Halopriming imparts tolerance to salt and PEG induced drought stress in sugarcane. Agric. Ecosyst. Environ. 134: 24–28.
Sparg, S. G., M. G. Kulkarni, and J. van Staden. 2006. Aerosol smoke and smoke-water stimulation of seedling vigor of a commercial maize cultivar. Crop Sci. 46: 1336–1340.
Suksoon, L., and K. Jaehyeun. 2000. Total sugars, α-amylase activity, and germination after priming of normal and aged rice seeds. Korean J. Crop Sci. 45: 108–111.
Wattanakulpakin, P., S. Photchanachai, S. Miyagawa, and K. Ratanakhanokchai. 2012. Loss of maize seed vigor as affected by biochemical changes during hydropriming. Crop Sci. 52: 2783–2793.
Yang, T., and B.W. Poovaiah. 2002. Hydrogen peroxide homeostasis: Activation of plant catalase by calcium/calmodulin. Proc. Natl. Acad. Sci. 99: 4097–4102.
Amjad, M., K. Ziaf, Q. Iqbal, I. Ahmad, M. A. Riaz, and Z. A. Saqib. 2007. Effect of seed priming on seed vigour and salt tolerance in hot pepper. Pak. J. Agri. Sci. 44: 408–414.
Andreoli, C., and A. A. Khan. 2000. Integration of physiological, chemical and biological seed treatments to improve stand establishment and yield of vegetables. Acta Hortic. 533: 31–38.
Bailly, C., A. Benamar, F. Corbineau, and D. Come. 1998. Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds. Physiol. Plant 104: 646–652.
Beauchamp, C., and I. Fridovich. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276–287.
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Brecht, J. K., S.A. Sargent, R.C. Hochmuth, and R.S. Tervola. 1990. Postharvest quality of supersweet (sh2) sweet corn cultivars. Proc. Fla. State Hort. Soc. 103: 283–288.
Cao, D., J. Hu, X. Huang, X. Wang, Y.Guan, and Z. Wang. 2008. Relationships between changes of kernel nutritive components and seed vigor during development stages of F1 seeds of sh2 sweet corn. J. Zhejiang Univ. Sci. B 9: 964–968.
Chaplin, M. F. 1986. Monosacharides In: M.F.C. Kennedy (ed.) Carbohydrate Analysis, a Practical Approach. Oxford, Washington DC: IRL Press, pp. 1–36.
Delouche, J. C., and C. C. Baskin. 1973. Accelerated aging techniques for predicting the relative storability of seed lots. Seed Sci. Technol. 1: 427–452.
Demiral, T., and I. Turkan. 2005. Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environ. Exp. Bot. 53: 247–257.
Don, R., ISTA Germination Committee. 2006. ISTA Handbook on Seedling Evaluation. Bassersdorf, Switzerland: International Seed Testing Association.
Esterbauer, H, and K. H. Cheeseman. 1990. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 186: 407–421.
Farooq, M., S. M. A. Barsa, and A. Wahid. 2006. Priming of field-sown rice seed enhances germination, seedling establishment, allometry and yield. Plant Growth Regul. 49: 285–294.
Flematti, G. R., E. L. Ghisalberti, K. W. Dixon, and R.D. Trengove. 2004. A compound from smoke that promotes seed germination. Sci. 305: 977.
George, D. L., M. L. Gupta, D. Tay, and I. G. M.A Parwata. 2003. Influence of planting date, method of handling and seed size on super sweet corn seed quality. Seed Sci. Technol. 31: 351–366.
Gholami, M., F. Mokhtarian, and B. Baninasab. 2015. Seed halopriming improves the germination performance of black seed (Nigella sativa) under salinity stress conditions. J. Crop. Sci. Biotechnol. 18: 21–26.
Goel A, A. K. Goel, and I. S. Sheoran. 2003. Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. J. Plant Physiol. 160: 1093–1100.
Góth, L. 1991. A simple method for determination of serum catalase activity and revision of reference range. Clin. Chim. Acta. 196: 143-152.
Guan, Y., J. Hu, X. Wang, and C. Shao. 2009. Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. J. Zhejiang Univ. Sci. B 10: 427–433.
Harris, D., A. Joshi, P. A. Khan, P. Gothkar, and P. S. Sodhi. 1999. On-farm seed priming in semi-arid agriculture: development and evaluation in maize, rice and chickpea in India using participatory methods. Exp. Agr. 35: 15–29.
Hartz, T. K., and J. Caprile. 1995. Germination of sh2 sweet corn following seed disinfestation, solid-matrix priming, and microbial seed treatment. HortSci. 30: 1400–1402.
Hsu, C. C., C. L. Chen, J. J. Chen, and J. M. Sung. 2003. Accelerated aging-enhanced lipid peroxidation in bitter gourd seeds and effects of priming and hot water soaking treatments. Sci. Hortic. 98: 201–212.
Hussain, S., M. Zheng, F. Khan, A. Khaliq, S. Fahad, S. Peng, J. Huang, K. Cui, and L. Nie. 2015. Benefits of rice seed priming are offset permanently by prolonged storage and the storage conditions. Scientific Reports Jan 29; 5:8101.
Jie, L., L. Gongshe, Q. Dongmei, L. Fangfang, and W. Enhua. 2002. Effect of PEG on germination and active oxygen metabolism in wild rye (Leymus chinensis) seeds. ACTA Pratacult. Sin. 11: 59–64.
Jothityangkoon, D., C. Ruamtakhu, S. Tipparak, S. Wanapat, and A. Polthanee. 2007. Wood vinegar enhances seed germination and seedling development of rice. P. 35–40. In Proc. of the 2nd Int. Conf. on Rice for the Future, Queen Sirikit National Convention Center, Bangkok, Thailand.
Kranner, I., R. P. Beckett, F. V. Minibayeva, and C. E. Seal. 2010. Tansley review: What is stress? Concepts, definitions and applications in seed science. New Phytol. 188: 655-673.
Kulkarni, M. G, S. G. Sparg, S. G., M. E. Light, and J. van Staden. 2006. Stimulation of rice (Oryza sativa L.) seedling vigour by smoke-water and butenolide. J. Agron. Crop Sci. 192: 395–398.
Lertrat, K., and T. Pulam. 2007. Breeding for increased sweetness in sweet corn. Int. J. Plant Breed. 1: 27–30.
Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426–428.
Mukhtar, T., M. Z. Kayani, and M.A. Hussain. 2013. Response of selected cucumber cultivars to Meloidogyne incognita. Crop Prot. 44: 13–17.
Murthy, U. M. N., P. P. Kumar, and W. Q. Sun. 2003. Mechanisms of seed ageing under different storage conditions for Vigna radiata (L.) Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition. J. Exp. Bot. 54: 1057–1067.
Nakano, Y., and K. Asada. 1987. Purification of ascorbate peroxidase in spinach chloroplasts; Its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiol. 28: 131–140.
Patade, V. Y., S. Bhargava, and P. Suprasanna. 2009. Halopriming imparts tolerance to salt and PEG induced drought stress in sugarcane. Agric. Ecosyst. Environ. 134: 24–28.
Sparg, S. G., M. G. Kulkarni, and J. van Staden. 2006. Aerosol smoke and smoke-water stimulation of seedling vigor of a commercial maize cultivar. Crop Sci. 46: 1336–1340.
Suksoon, L., and K. Jaehyeun. 2000. Total sugars, α-amylase activity, and germination after priming of normal and aged rice seeds. Korean J. Crop Sci. 45: 108–111.
Wattanakulpakin, P., S. Photchanachai, S. Miyagawa, and K. Ratanakhanokchai. 2012. Loss of maize seed vigor as affected by biochemical changes during hydropriming. Crop Sci. 52: 2783–2793.
Yang, T., and B.W. Poovaiah. 2002. Hydrogen peroxide homeostasis: Activation of plant catalase by calcium/calmodulin. Proc. Natl. Acad. Sci. 99: 4097–4102.