Isolation, Screening and Confirmative Identification of High Hypocrellin A-Producing Shiraia bambusicola Isolates

บทคัดย่อ

The main objective of this study was to obtain high hypocrellin A (HA)-producing isolates of fungus Shiraia bambusicola. Fungal cultures were obtained from stromata of presumably S. bambusicola, found on Pleioblastus sp., Brachystachyum sp. and Phyllostachys sp. bamboos by isolation of single spore, multispores and stroma tissue. Appropriate HA sampling time and screening procedure were elucidated by selecting representative isolates to determine their mycelia biomass and HA content. Both morphological characteristics and ITS sequences were analyzed for confirmative identification of the isolates. Altogether 32 isolates of the fungus were obtained from Anhui and Zhejiang province of southern China in this study. All isolates were found to produce HA but its content in the single spore isolates were much lower than that of the isolates from multispore and stroma tissue during the sampling time at the 11th to 13th days of fermentation. The GZAAS2.0629 isolate was screened out as the highest HA yielding. It was confirmatively identified as S. bambusicola based on morphological and ITS sequence analysis.