Effect of organic trace mineral on gene expression of antioxidant enzyme and meat quality responsible enzyme in young chick

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Thanapol Puangmalee
Wirawan Junlapho
Yuwares Ruangpani

บทคัดย่อ

Trace minerals play every role that associated with metabolism process and necessary for the growth of animals. In general, mineral premixes are produced in salts form of inorganic minerals to full fill mineral requirement of animal. However, inorganic minerals are relatively low availability in broilers. The organic mineral form has higher availability than the inorganic minerals. It can be used in lower doses without affecting production performance of broilers. This research is aimed to investigate the effects of organic mineral as a total replace to inorganic mineral on growth performance, antioxidant function and meat quality responsible enzyme on gene expression by using real-time PCR. Eight hundred, one day old, male broiler chicks of Ross 308 were divided into 4 dietary treatments including: T1) Positive control (PC)  Diet with inorganic premix, T2) PC + organic minerals 500 g/tonne, T3) diet without inorganic Zn, Cu, Mn, Se, I and Fe in premix + organic minerals 750 g/tonne and T4) T3 + selenium yeast 200 g/ton. The trail ran between 1 and 21 days of age. Growth performance was not significantly different between treatments in terms of feed intake and mortality. For body weight gain and ADG, T2 and T1 showed no significant difference. However, these treatments produced higher body weight gain and ADG than T3 and T4 (P<0.05). The treatments showed a similar result on feed conversion ratio. The experiments on gene expression, muscle and liver samples immediately collected and perform to quantify the mRNA expression level of gene involved in meat quality and antioxidant function by real-time PCR. Supplementation of broiler diets with organic form of mineral was increased mRNA expression of a gene involved the glutathione redox cycle including glutathione peroxidase and glutathione S-transferase-α (P<0.05). Furthermore, organic trace mineral was decreased mRNA expression of a gene involved in meat quality to show that there is an increase in muscle development (P<0.05). Blood sample for Antioxidant enzyme measurement was selected form RNA expression measurement. GPx enzyme was measured by enzyme-linked immune sorbent assay (ELISA) kit. The concentration of glutathione peroxidase in antioxidant enzyme measurement was increased same with the result of mRNA expression (P<0.05). Thus, replacement of organic trace mineral supplements in broiler diets may be improve growth rate and meat quality for trace mineral supplementation of feed mixtures for poultry.

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