Role of arbuscular mycorrhizal fungi (AMF) in cocoa (Theobroma cacao L.) seedlings growth

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Ratiya Pongpisutta
Chainarong Rattanakreetakul
Tharnrat Kaewgrajang


Arbuscular mycorrhiza (AM) fungi are mutualistic symbiotic with plant roots of around 80% vascular plants, including corn and cocoa. This study aimed to identify and inoculate of AM fungi to cocoa seedlings growth. Cocoa seedlings were planted on polybag with sterilized peat-moss for 20 weeks in green house. Two species of AM fungi, Glomus constrictum and Acaulospora spinosa were identified by using morphological and molecular markers. Results of molecular method showed that PCR technique with ALF01 and NDL22 primers were detected in all isolates with sequence analysis around 350bp PCR band. Interaction between AM fungi and low phosphate fertilizer had significantly effect on growth of cocoa seedlings concerning to seedling height, leaves number and stem diameter. Glomus constrictum and 2 g of NPK showed higher response in all parameters than without AM fungi and NPK fertilizer.


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