Identification into species of the fungal fruit rot disease on Nam Dok Mai Si Tong mango for export

Abstract

Nam Dok Mai Si Tong mango is an important economic fruit in Thailand. The major problem in mango production for export caused by postharvest fruit rot disease. We performed collected mango fruit rot disease of Nam Dok Mai Si Thong from Banhad district Khon Kaen province. Thirsty disease samples were isolated using tissue transplanting method. The result showed that twenty isolates of fungi were obtained. Pathogenicity tests indicated that all of these fungal isolates were pathogenic on mango fruits and isolate NDM 8 was the most aggressive pathogen with the lesion size on mango fruit of 100.73 mm. Eleven isolates of fungal pathogen were selected for identifying into species based on morphological characterization and molecular technique. The DNA fragment of internal transcribe spacer of rDNA (ITS1-5.8S-ITS2) and translation elongation factor 1-alpha (EF1-α) gene were amplified by PCR technique. Identification of fruit rot pathogenic fungi were belong to five species as Lasiodiplodia theobromae (NDM5, NDM7, NDM8, NDM17, NDM19, NDM20), Lasiodiplodia pseudotheobromae (NDM16, NDM18), Botryosphaeria dothidea (NDM10), Colletotrichum gloeosporioides (NDM12) and Diaporthe pascoei (NDM13). The result of this study suggests can be applied as a strategy to improve the efficiency of fruit rot diseases control for Nam Dok Mai Si Tong mango in the field.