Study of fungal metagenomics and potential to produce secondary metabolites fungus isolated from Perionyx sp. 1 gut
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Abstract
The aim of this research was to identify the fungi species from Thai economic earthworm gut (Perionyx sp. 1) and testing on the efficiency to produce secondary metabolites (IAA, GA, Hydroxamate siderophore). As metagenomics level, revealed 3 phyla 8 classes 32 genera and 49 species. On the other hand, the conventional method could collect 11 different fungal isolates using ITS1. i.e., Talaromyces wortmannii (YL1), Chaetomium globosum (YL2), T. indioticus (YL3), T. angelicus (RL1), Trichoderma sp. (RL2), Aspergillus niger (RL3), Penicillium citrinum (Pe1), A. fumigatus (Pe2), A. flavus (Pe3), Humicola sp. (Pe5) and Fusarium sp. (Pe6). Three secondary metabolites (IAA, GA, Hydroxamate siderophore) were examined. The result showed that the PDBt added 0.4% L-tryptophan could produce IAA as 36.58 µg/ml higher than without. The GA showed the metabolite secretion related to IAA. However, the average of all fungal isolates could produce hydroxamate as 180.993 µg/ml and the RL3 isolate (Aspergillus niger) was the best fungi producing hydroxamate as 1,629.90 ± 0.0128 µg/ml. Furthermore, RL3 supernatant mixed iron solution (1:103) could stimulate the growth of green pea shoot more than outers, all of which confirm that within the intestinal gut of Perionyx sp.1 compose of effective fungi which can stimulate plant growth.
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