Screening for effective Nakhon Ratchasima province isolates of entomopathogenic fungi for controlling larvae and pupae of housefly (Musca domestica L.)
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Abstract
The housefly, Musca domestica L. is an insect pest that acts as a vector for many pathogenic diseases and cause nuisance in both people and animals.The present study was conducted to evaluate the virulence and efficiency of different local isolates of entomopathogenic fungi to control housefly in laboratory conditions. Entomopathogenic fungi were collected and isolated from soil in Nakhon Ratchasima province by baiting technique using yellow mealworm larvae, Tenebrio molitor L., as bait. Total of 45 isolates of entomopathogenic fungi with the 41 isolates of Metarhizium spp. and 4 isolates of Beauveria bassiana were found. The entomopathogenic fungal bioassay comparison was carried out to examine the virulence effecting of housefly (2nd instar of larvae and pupae). Total of 34 and 13 isolates out of 45 isolates had effective infection of larvae (4.44 – 97.78%) and pupae (2.22 – 97.78%), respectively. When compared at conidial concentrations of 1×108 conidia/ml, Metarhizium spp. isolates NM_SS 9/2 had the highest infection with 86.67% on housefly larvae, which was non-significantly difference (P>0.05) from Metarhizium spp., isolate NM_BAL 7/1 (71.11%) and NM_NS 7/2 (68.89%). Meanwhile, Metarhizium spp. isolate NM_CLPK 4/1 had ability of infection to pupae 60.00%, followed by NM_SN 2/1 with 57.78%. This present study suggested that entomopathogenic fungi isolated from Nakhon Ratchasima province had the efficiency to control housefly and can be applied in controlling and preventing housefly in animal farm conditions.
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References
คม สุคนธสรรพ์ และกาบแก้ว สุคนธสรรพ์. 2548. แมลงที่มีความสำคัญทางการแพทย์ในประเทศไทย เล่มที่ 1. บริษัท เชียงใหม่ดิจิตอลเวิร์คส จำกัด: เชียงใหม่.
ศิวิลัย สิริมังครารัตน์. 2557. โรควิทยาของแมลงและการประยุกต์ใช้. สาขากีฏวิทยา คณะเกษตรศาสตร์ มหาวิทยาลัยขอนแก่น.
สถาบันวิจัยวิทยาศาสตร์และสาธารณสุข. 2553. ชีววิทยาและการควบคุมแมลงที่เป็นปัญหาสาธารณสุข. บริษัทหนังสือดีวันจำกัด: นนทบุรี.
อาคม สังข์วรานนท์. 2538. กีฏวิทยาทางการแพทย์. โรงพิมพ์สหมิตรพริ้นติ้ง, นนทบุรี.
Abbott, W.S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology. 18: 265-267.
Abdel-Raheem, A.M., and B.M. Eldafrawy. 2016. Effect of entomopathogenic fungi as biocides against house fly, Musca domestica L. (Diptera: Muscidae). Journal of Plant Protection and Pathology. 7: 633–636.
Acevedo, G.R., M. Zapater, and A.C. Toloza. 2009. Insecticide resistance of house fly, Musca domestica (L.) from Argentina. Journal of Parasitology Research. 105: 489-493.
Acharya, N., E.G. Rajotte, N.E. Jenkins, and M.B. Thomas. 2014. Potential for biocontrol of house flies, Musca domestica, using fungal biopesticides. Biocontrol Science and Technology. 25: 513-524.
Archana, M., P.E. D’Souza, and J. Patil. 2017. Efficacy of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) on developmental stages of house fly, Musca domestica. Journal of Parasitic Diseases. 41(3): 782–794.
Azzam, S, and E. Hussein. 2002. Toxicities of several insecticides to the house fly Musca domestica from different regions in Jordon. Sarhad Journal of Agriculture. 18: 69–75.
Barson, G., N. Renn, and A.F. Bywater. 1994. Laboratory evaluation of six species of entomopathogenic fungi for the control of the house fly (Musca domestica L.), a pest of intensive animal units. Journal of Invertebrate Pathology. 64: 107-113.
Bidochka, M.J., J.E. Kasperskl, and G.A.M. Wild. 1998. Occurrence of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana in soil from temperate and near northern habitats. Canadian Journal of Botany. 76: 1198-1204.
Bugti, G.A., W. Bin, S.A. Memon, G.K., and M.A. Jaffar. 2020. Entomopathogenic fungi: factors involved in successful microbial control of insect pests. Journal of Entomology. 17: 74-83.
Cantwell. G.E., A.J. Noppi, and J.G. Stoffolano. 1976. Embryonic and postembryonic development of the house fly. Agricultural Research Service. United State Department of Agricultural. Washington D.C.
Farooq, M., and S. Freed. 2016. Infectivity of housefly, Musca domestica (Diptera: Muscidae) to different entomopathogenic fungi. Brazilian Journal of Microbiology. 47: 807-816.
Geden, C.J. 2012. Status of biopesticides for control of house flies. Journal of Biopesticides. 5: 1–11.
Humber, R.A. 2005. Entomopathogenic fungal identification. Plant Protection Research Unit US Plant, Soil & Nutrition Laboratory. Las Vegas, America.
Ibrahim, A.A., M.I. Hassan, A.I. Hasaballah, M.A. Fouda, and G.M. Omar. 2016. Evaluation of some entomopathogenic fungi against larvae, pupae and adults of house fly, Musca domestica L. Journal of Nuclear Technology in Applied Science. 4: 103-112.
Iqbal, W., M.F. Malik, M.K. Sarwar, I. Azam, N. Iram, and A. Rashda. 2014. Role of house fly (Musca domestica, Diptera : Muscidae) as a disease vector; a review. Journal of Entomology and Zoology. 2: 159–163.
Jaronski, S.T. 2009. Ecology factors in the inundate use of fungal entomopathogens. Biocontrol. 5: 159-185.
Khan, M.F., and S.M. Ahmed. 2000. Toxicity of crude neem leaf extract against housefly (Musca domestica L.) adults as compared with DDVP. Turkish Journal of Zoology. 24: 219–223.
Merdan, B.A. 2012. Bacillus thuringiensis as a feed additive to control Musca domestica associated with poultry house. Journal of Basic and Applied Zoology. 65: 83–87.
Mishra, S., and A. Malik. 2012. Comparative evaluation of five Beauveria isolates for housefly (Musca domestica L.) control and growth optimization of selected strain. Parasitology Research. 111: 1937–1945.
Mishra, S., P. Kumar, and A. Malik. 2011. Adulticidal and larvicidal activity of Beauveria bassiana and Metarhizium anisopliae against house fly, Musca domestica (Diptera : Muscidae), in laboratory and simulated field bioassays. Parasitology Research. 108: 1483–1492.
Mohammed, A.A. 2018. Evaluation the efficacy of Metarhizium anisopliae and some plant extracts in controlling the housefly, Musca domestica in laboratory conditions. Kufa Journal for Agricultural Science. 10: 1-17.
Mostafa, A.A., and A.B. Zayed. 1999. Resistance of Musca domestica (L) (Diptera: Muscidae) from Gamsa City to some insecticides. Egyptian Society of Parasitology. 29: 193–201.
Mwamburi, L.A., M.D. Laing, and R.M. Miller. 2019. External development of the entomopathogenic fungus Beauveria bassiana on the Housefly (Musca domestica). The Proceedings of the National Academy of Sciences, India, Section B: Biological Sciences. 89(3): 833–839.
Samson, R.A., H.A. Evans, and J.P. Latge. 1998. Atlas of entomopathogenic fungi, Springer, Berlin.
Sharififard, M, M.S. Mossadegh, and B. Vazirianzadhe. 2012. Effects of temperature and humidity on the pathogenicity of the entomopathogenic fungi in control of the house fly, Musca domestica L. (Diptera: Muscidae) under laboratory conditions. Journal of Entomology. 9: 282–288.
Sharififard, M, M.S. Mossadegh, B. Vazirianzadhe, and A.Z. Mahmoudabadi. 2011. Laboratory pathogenicity of entomopathogenic fungi, Beauveria bassiana (Bals.) Vuill. and Metarhizium anisoplae (Metch.) Sorok. to larvae and adult of house fly, Musca domestica L. (Diptera: Muscidae). Asian Journal of Biological Sciences. 4: 128–137.
Uma Devi, K., J. Padmavathi, U.M. Rao, C. Khan, A.P. Akbar, and C.M. Murali. 2008. A study of host specificity in the entomopathogenic fungus Beauveria bassiana (Hypocreales, Clavicipitaceae). Biocontrol Science and Technology. 18: 975-989.
Watson, D.W., C.J. Geden, S.J. Long, and D.A. Rutz. 1995. Efficacy of Beauveria bassiana for controlling the house fly and stable fly (Diptera: Musciadae). Biological control. 5: 405–411.
