Polyphasic characterization of Xanthomanas axonopodis pv. citri isolated from lemon and Leech Lime in Maha Sarakham province

Abstract

The objective of this study was undertaken to characterize Xanthomonas axonopodis pv. citri (Xac) by a polyphasic approach. Twenty-nine isolates of bacterial samples were isolated from infected leaf and fruit of lemon and leech lime. All isolates were used to pathogenicity test on lemon, leech lime and tangerine plant. The results showed 25 isolates showed typical symptoms and indicated that all bacterial isolates were Xac . Twelve isolates of Xac were selected and used to characterize the original host plant by biochemical and physiological characterization. The results showed, their biochemical and physiological properties were not related to the original host plant. Moreover, the results from protein pattern analysis, 16s rDNA gene analysis by ARDRA technique and REP-PCR fingerprinting analysis showed similarities agree with those biochemical and physiological results. After combining all data and analysis by using polyphasic method, the results showed these polyphasic characterization could classify all selected Xac isolates to original host plant.