Indentification of interspeccific hybrids between Centrosema pascuorum cv. Cavacade with Centrosema pubescens and Centrosema macrocarpum using RAPD technique

Abstract

Study on mechanism and methods to overcome barriers in interspecific crossing between Centrosema passcuorum cv. Cavalcade with Centrosema pubescens and Centrosema macrocarpum was carried out at Khon Kean University during September 2002-November 2004. The interspecific hybrids from both cross and reciprocal cross were quite similar until we could not classified from the mother plants. RAPD technique was applied for their identification with the modified method of Keb-llanes et al (2002). DNA extraction give the good DNA solution of all hybrid and inbred progeny in this experiment. The combination of 2 primers gave difference banding pattern of the parents and hybrid and reciprocal hybrids.