Assessment of genome and genetic diversity in (Helianthus tuberosus L.) with ISSR markers

Abstract

Jerusalem artichoke (Helianthus tuberosus L.) is native of North America. The high-fructose syrupsderived from the tubers may be used primarily as sweeteners in the food industry, also for the production of ethanoland be suitable for use as cattle fodder. There was no genome and genetic diversity information of this plantspecies. Thus, the aim of this study was to analyze the genetic diversity of H. tuberosus germplasm collected fromthe USA, Germany, France, Russian Federation and Canada using Inter Simple Sequence Repeat (ISSR) techniquewith primers containing microsatellites which are ubiquitous in plant genomes. A total of 24 primers, containingdifferent Simple Sequence Repeat (SSR) motifs, were tested for potential amplification. Six primers produced 552repeatable bands in the range of 200-1500 bp, of which 344 were polymorphic PCR products and 47 variety-specificbands whereby flanking sequence 3' anchored primer gave more ISSR-PCR products than that of 5' anchoredprimers. The ISSR profiles of H. tuberosus generated by (GATA)2 (GACA)2, HVH (TCC)5 and (GA)6 YT primersshowed that these repeats are abundant in the H. tuberosus genome. This genome which distinguished high tuberyield accessions from the others and also separated cultivated accessions from wild H. tuberosus.