Effects of tyrosine and plant growth regulators on growth and development of Gloriosa superba Linn. In vitro

Abstract

Effects of tyrosine and plant growth regulators on rhizome cultures were carried out on modifiedMurashige and Skoog’s medium. The sterile rhizomes were cut and cultured on MS medium supplemented with 1mg./l. 2,4-D, 1 mg./l. BA and various concentrations of tyrosine under the light and dark condition. The experimentaldata showed that the highest percentage of callus formation 100% was on the medium added with 50 mg./l. tyrosinein the dark condition and 85% in the light condition. The average fresh weight of callus cultured in the dark for16 weeks was 6.25 g. Shoot induction was performed using the combination of NAA and BA for 36 weeks. Theexperimental data revealed that the shoot regeneration from callus was 100% on the medium added with 4 mg./l. BAand 4 mg./l. NAA. Comparison among various concentrations of BA and NAA, it was shown that shoot formationwas 83% and 50% on the medium containing 4 mg./l. BA and 4 mg./l. kinetin, respectively. The chromosomenumber from root tip obtained from tissue culture was 2n = 22. (Keywords: glory lily, tissue culture, tyrosine,Liliaceae, rhizome)