Preliminary investigation of Emericella nidulans's culture and extracts against anthracnose pathogen of Vanilla albida Blume

Abstract

The effect of Emericella nidulans against anthracnose pathogen of Vanilla albida Blume caused byColletotrichum gloeosporioides (Penz). Eight isolates of C. gloeosporioides were isolated by tissue transplantingtechnique and were tested for their pathogenicity. The results showed that isolate VA8 gave the highest virulence fordisease incidence. E. nidulans was isolated from fallen leaves of V. albida and tested against mycelial growth andspore production of C. gloeosporioides VA8 by bi-culture and crude extract methods. Bi-culture test showed thatE. nidulans could inhibit mycelial growth and spore production at 29.17% and 31.90%, respectively. Crude extractswere extracted from E. nidulans with hexane, ethyl acetate and methanol. Crude extract which was extracted withhexane gave the best mycelial growth inhibition and spore production at the concentration of 1,000 μg/ml and theeffective dose (ED50) were 1,450 μg/ml and 0.0006 μg/ml, respectively.