In Vitro shoot multiplication and Root Induction of Bambusa nana

Abstract

The effects of plant growth regulators and concentrations on shoot multiplication and root induction ofBambusa nana were investigated. The single nodes were cultured on MS media supplemented with 0, 2, 4, 6, 8 and10 mg/l BA for 3 weeks to induce shoot growth. 71-90% of cultured nodes could develop into shoots. The generatedshoot number and the shoot length of each treatment were not significantly different. For shoot proliferation, thepropagules of 4 shoots were cultured on MS medium supplemented with 4 mg/l BA and 0, 0.1, 0.3 or 0.7 mg/l IBAfor 3 weeks. The highest number of shoots produced at 2.8 shoots occurred on the MS medium supplemented with4 mg/l BA and 0.3 mg/l IBA. However, the total number of shoots, the number of shoot produced and the shootlength were not significantly different among the treatments. The result of root induction on MS media supple-mented with 4 mg/l BA and 9-30 mg/l NAA revealed that rooting percentage, number of roots and root length weresignificantly different among the treatments. The propagules cultured on MS medium supplemented with 4 mg/l BAand 15 mg/l NAA gave the greatest of rooting percentage, the number of roots and the root length of 81%, 3.1 rootsand 2.36 cm, respectively after 8 weeks of culture.