Resistance to anthracnose disease of chili progressive lines derived anthracnose resistant genes from PBC80 and PBC932 to three Colletotrichum species
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Abstract
Chili production in Thailand have been lost by a various of Colletotrichum spp. The disease affected to yield and fruit quality of both fresh and dried chili products. Therefore, the experiment aimed to evaluate the anthracnose resistant cultivars from 65 chili progressive lines (accuired anthracnose resistance gene from chili PBC932 and PBC80) compared to commercial cultivars and assess resistance to anthracnose of three species of three Colletotrichum species were C. cappsici (CC), C. acutatum (CA), and C. gloeosporoides (CG) at mature green (G) and ripe red (R) fruit stages. The experiments were carried out by randomized complete block design (RCBD) with three replications and ten fruits per replication. The screening method was performed by the microinjection technique with the spore suspension concentration of 5 × 105 spores/mL with 2 µL/fruit. The disease severity was evaluated by lesion diameter at day 5th after inoculation. The results showed that KM6-4-1 line was resistant to three Colletotrichum species at both mature green (G) and ripe red (R) fruit stages, while KM6-2-1 and KM1-9-1 lines were resistant to CA-G, CA-R, CC-G, CC-R and CG-G. However, some varieties were resistant to the single trait, 11 lines were resistant to CA-G , 36 lines were resistant to CA-R, 42 lines were resistant to CG-G , 29 lines were resistant to CG-R, 15 lines were resistant to CC-G and 20 lines were resistant to CC-R. In addition, CA specie showed aggressive more than others two species. Therefore, the resistant lines will be evaluated with the agronomic traits and yield for developing a parental line for producing a commercial F1-hybird variety against anthracnose disease.
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References
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