Detection of genotype patterns at IGF-I, SCD, FABP4 and FASN genes in Thai native Cattle and Brahman
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Abstract
Thai native cattle and Brahman are common breeds for smallholder farmers. The management of breeding selection has been involved for improving the productive performance, but there is a lack information of pedigree records and data collection of important economic traits. At the same time, molecular genetics technology has a major role to find genetic markers for the development of growth traits and meat quality in cattle which could promote value added of cattle production for farmers. Hence, the purpose of this study was to investigate the genotype patterns of IGF-I, SCD, FABP4, and FASN genes in 110 Kho-Isaan cattle and 50 Brahman by Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP). The result found that two genotypes were identified in the IGF-I gene (CT and CC) and the FASN gene (AG and GG). For the SCD gene, three genotypes were identified: CC, CT, and TT. For the FABP4 gene, only one genotype (GG) was found. The IGF-I has C allele frequencies with the range of 0.930-1.000, while the SCD gene has T allele frequencies with the range of 0.720-0.775 and the FASN gene has a G allele frequency of 0.970-0.995 in Kho-Isaan cattle and Brahman. For the FABP4 gene, the G allele frequency was 1.000 in both breeds. The HO, HE, uHE, and PIC analyses showed moderate polymorphism in SCD genes and low polymorphism in IGF-I and FASN genes, and the resulting equilibrium of genes revealed IGF-I, SCD, and FASN genes were in equilibrium according to the rules of Hardy–Weinberg Equilibrium (HWE). Therefore, the SCD gene has the potential to be studied in association with meat quality and fatty acid composition to develop the production of beef cattle in the future.
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