Biological control agents as alternative of chemical insecticides in melon greenhouseBiological control agents as alternative of chemical insecticides in melon greenhouse
Article Sidebar
Main Article Content
Abstract
Melon is an important fruit crop in Thailand. Biological control by microorganisms is a pest management method intended for organic agriculture, good agricultural practice (GAP), and crop export. This study aimed to evaluate the biocontrol effectiveness of three biocontrol agents (BCAs), including Beauveria bassiana BCC 2660, Metarhizium sp. BCC 4849 and Trichoderma asperellum TBRC 4734 for pest and disease control in melon plantation in greenhouse, compared with synthetic pesticides, and to investigate clothianidin residues in melon and soil. Three damaging pests in melon included red mite (Eutetranychus africanus), tobacco whitefly (Bemisia tabaci), and melon thrip (Thrips palmi). The populations of the three pests on the undersides of the leaves in the BCA treatment decreased by 34.8, 0.3, 18.7% and 66, 0.3, 8.9%, respectively, compared with those in the BCA-synthetic and synthetic treatments. On the yellow traps, the whiteflies and thrips were found. The whitefly and thrip populations in the BCA treatment also decreased by 6.5, 17.7% and 7.3, 21.5%, respectively, compared with those in the BCA-synthetic and synthetic treatments. Furthermore, the BCAs also enriched the soil organic matter by 15%, compared with the synthetic treatment, which potentially enhances the nutrient availability for crop growth and development. For the synthetic treatment, we detected 0.023 and 1.607 mg/kg clothianidin residues in melon fruits and in the soil, respectively using LC-MS method. For synthetic-BCA combined treatment, there were markedly low residues at 0.0008 and 0.258 mg/kg in melon and soil, respectively. There were no chemical residues found in the BCA treatment. Our data indicate that the BCAs can be used effectively as an alternative of synthetic pesticides and would have the potential in pest management for organic agriculture and GAP.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
นาวิน สุขเลิศ, จิราพร กุลสาริน, ไสวบรูณพานิชพันธุ์ และวีรเทพ พงษ์ประเสริฐ. 2559. ประสิทธิภาพของสารชีวภัณฑ์เชื้อรากำจัดแมลงในการควบคุมด้วงหมัดผักแถบลายในเบบี้ฮ่องเต้บนพื้นที่สูงของจังหวัดเชียงใหม่. วารสารเกษตร. 32(2): 171-180.
วัฒนา จารณศรี, มานิตา คงชื่นสิน, เทวิน กุลปิยะวัฒน์ และพิเชฐ เชาวน์วัฒนวงศ์. 2544. ไรศัตรูพืชและการป้องกันกำจัด. กรมวิชาการเกษตร กองกีฏและสัตววิทยา กลุ่มงานวิจัยไรและแมงมุม. กรุงเทพฯ.
อลงกรณ์ อำนวยกาญจนสิน, รัศมี หวะสุวรรณ, เชษฐ์ธิดา ศรีสุขสาม, ธงชัย ตั้งใจดี, กัลยารัตน์ รัตนะจิตร, เสาวนีย์ ปานประเสริฐกุล,ยุวนันท์ สันติทวีฤกษ์, กฤษดา บำรุงวงศ์, สุชาดา มงคลสัมฤทธิ์, เจนนิเฟอร์ เหลืองสะอาด และมรกต ตันติเจริญ. 2564. ภาพลักษณ์ของราแมลงและการใช้ประโยชน์ในสังคมไทย. วารสารมนุษยศาสตร์ปริทรรศน์. 43(1): 111-123.
Abdallah, O.I., R.M. Abd El-Hamid, and E.H. Abdel Raheem. 2019. Clothianidin Residues in Green Bean, Pepper and Watermelon Crops and Dietary Exposure Evaluation Based on Dispersive Liquid-Liquid Microextraction and Lc–Ms/Ms. Journal of Consumer Protection and Food Safety. 14(3): 293-300.
Aktar, M.W., D. Sengupta, and A. Chowdhury. 2009. Impact of Pesticides Use in Agriculture: Their Benefits and Hazards. Interdisciplinary toxicology. 2(1): 1-12.
Akutse, K.S., F.M. Khamis, F.C. Ambele, J.W. Kimemia, S. Ekesi, and S. Subramanian. 2020. Combining Insect Pathogenic Fungi and a Pheromone Trap for Sustainable Management of the Fall Armyworm, Spodoptera Frugiperda (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology. 177: 107477.
Al-mazra'awi, M., A. Al-Abbadi, M. Shatnawi, and M. Ateyyat. 2009. Effect of Application Method on the Interaction between Beauveria Bassiana and Neem Tree Extract When Combined for Thrips Tabaci (Thysanoptera: Thripidae) Control. Journal of Food Agriculture and Environment. 7: 869-873.
Amnuaykanjanasin, A., J. Jirakkakul, C. Panyasiri, P. Panyarakkit, P. Nounurai, D. Chantasingh, and M. Tanticharoen. 2013. Infection and colonization of tissues of the aphid Myzus persicae and cassava mealybug Phenacoccus manihoti by the fungus Beauveria bassiana. Journal of the International Organization for Biological Control. 58(3): 379-391.
Benítez, T., A.M. Rincón, M.C. Limón, and A.C. Codón. 2004. Biocontrol mechanisms of Trichoderma strains. International microbiology: the official journal of the Spanish Society for Microbiology. 7(4): 249-260.
Birkhofer, K., T.M. Bezemer, J. Bloem, M. Bonkowski, S. Christensen, D. Dubois, and S. Scheu. 2008. Long-term organic farming fosters below and aboveground biota: Implications for soil quality, biological control and productivity. Soil Biology and Biochemistry. 40(9): 2297-2308.
Bommuraj, V., Y. Chen, H. Klein, R. Sperling, S. Barel, and J.A. Shimshoni. 2019. Pesticide and trace element residues in honey and beeswax combs from Israel in association with human risk assessment and honey adulteration. Food Chemistry. 299: 125123.
Bonmatin, J.M., I. Moineau, R. Charvet, C. Fleche, M.E. Colin, and E.R. Bengsch. 2003. A LC/APCI-MS/MS Method for Analysis of Imidacloprid in Soils, in Plants, and in Pollens. Analytical Chemistry. 75(9): 2027-2033.
Bray, R.H., and L.T. Kurtz. 1945. Determination of Total Organic and Available Forms of Phosphorus in Soils. Soil Science. 59(1): 39-46.
Carter, M.R., and E.G. Gregorich. 2008. Soil sampling and methods of analysis. European Journal of Soil Science. 59(5): 1010-1011.
Castineiras, A., J.E. Peña, R. Duncan, and L. Osborne. 1996. Potential of Beauveria Bassiana and Paecilomyces Fumosoroseus (Deuteromycotina: Hyphomycetes) as Biological Control Agents of Thrips Palmi (Thysanoptera: Thripidae). The Florida Entomologist. 79(3): 458-461.
Hano, T., K. Ito, N. Ohkubo, H. Sakaji, A. Watanabe, K. Takashima, and K. Mochida. 2019. Occurrence of neonicotinoids and fipronil in estuaries and their potential risks to aquatic invertebrates. Environmental Pollution. 252: 205-215.
Harman, G.E., C.R. Howell, A. Viterbo, I. Chet, and M. Lorito. 2004. Trichoderma species - opportunistic, avirulent plant symbionts. Nature Reviews Microbiology. 2(1): 43-56.
Mahmood, I., S.R. Imadi, K. Shazadi, A. Gul, and K.R. Hakeem. 2016. Effects of Pesticides on Environment. Plant Soil and Microbes. 1: 253-269.
Palumbo, J.C., and D. Kerns. 1998. Melon Insect Pest Management in Arizona. University of Arizona, College of Agriculture and Life Sciences. IPM Series. 11: 1-7.
Rebecca B., United States. Natural Resources Conservation Service., National Soil Survey Center (U.S.)., and National Soil Survey Laboratory (U.S.). 2004. Soil survey laboratory methods manual. United States Dept. of Agriculture, Natural Resources Conservation Service. Washington, D.C.
Tabara, M., Y. Nagashima, K. He, X. Qian, K.M. Crosby, J. Jifon, G.K. Jayaprakasha, B. Patil, H. Koiwa, H. Takahashi, and T. Fukuhara. 2021. Frequent asymptomatic infection with tobacco ringspot virus on melon fruit. Virus research. 293: 198266.
Walkley, A., and I.A. Black. 1934. An Examination of the Degtjareff Method for Determining Soil Organic Matter, and a Proposed Modification of the Chromic Acid Titration Method. Soil Science. 37(1): 29-38.
Wang, A., G. Mahai, Y. Wan, Y. Jiang, Q. Meng, W. Xia, and S. Xu. 2019. Neonicotinoids and carbendazim in indoor dust from three cities in China: Spatial and temporal variations. Science of The Total Environment. 695: 133790.
Wang, X., L. Xue, S. Chang, X. He, T. Fan, J. Wu, J. Niu, and B. Emaneghemi. 2019. Bioremediation and Metabolism of Clothianidin by Mixed Bacterial Consortia Enriched from Contaminated Soils in Chinese Greenhouse. Process Biochemistry. 78: 114-122.
Wasuwan, R., N. Phosrithong, B. Promdonkoy, D. Sangsrakru, C. Sonthirod, S. Tangphatsornruang, S. Likhitrattanapisal, S. Ingsriswang, C. Srisuksam, K. Klamchao, M. Suksangpanomrung, T. Hleepongpanich, S. Reungpatthanaphong, M. Tanticharoen., and A. Amnuaykanjanasin. 2022. The Fungus Metarhizium Sp. Bcc 4849 Is an Effective and Safe Mycoinsecticide for the Management of Spider Mites and Other Insect Pests. Insects. 13(1): 42.
Zhang, Q., C. Shan, W. Song, W. Cai, F. Zhou, M. Ning, and F. Tang. 2021. Transcriptome Analysis of Starch and Sucrose Metabolism Change in Gold Queen Hami Melons under Different Storage Temperatures. Postharvest Biology and Technology. 174: 111445.
Zimmermann, G. 1993. The entomopathogenicf ungus Metarhizium anisopliaeand its potential as a biocontrol agent. PesticideScience. 37(4): 375-379.
